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FL-fibulin-3-V5 pcDNA4

Plasmid

#29701

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
HA-RNF34

Plasmid

#119938

Purpose

RING finger protein 34 expression (HA-tag at the N-terminus)

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only
pSLC-242

Plasmid

#73189

Purpose

Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)

Use

Tags

Expression

Bacterial

Mutation

Promoter

chloramphenicol promoter

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pL0_AC-H4

Plasmid

#202122

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AC-P49

Plasmid

#202120

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Susd4(3.7)-EGFP-sv40

Plasmid

#184413

Purpose

AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

Depositor

Marylka Yoe Uusisaari

Article

Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

Insert

Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Mouse Susd4 gene

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Craf S338A

Plasmid

#118335

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf S338A (RAF1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S338A

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCSN061

Plasmid

#101725

Purpose

Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

Depositor

Rene Verwaal

Article

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Inserts

S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Heterologous TEF1 promoter from A. gossypii. and …

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
EE-mut SV40 luc

Plasmid

#8460

Depositor

Bob Weinberg

Article

Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

Insert

human cyclin E promoter fragment, mutant (CCNE1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

E2F sites I, II, and III mutated.

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pLX311 LacZ

Plasmid

#117731

Purpose

Open reading frame vector encoding LacZ

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

LacZ

Use

Lentiviral

Tags

2X V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK102

Plasmid

#110574

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

T7 promoter with RBS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK124

Plasmid

#110583

Purpose

BTK Type 2 promoter for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

CP25 promoter with lac operator and RBS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRetro-XT-PAK2-CFP(puro)-L107F

Plasmid

#81180

Purpose

Retroviral vector for expression of kinase active PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Expression

Mutation

L107F

Promoter

P-tight TRE

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pQnl_tet

Plasmid

#191355

Purpose

Clostridium expression vector with TetR-repressed NanoLuc expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

PGusA2-TetO2/1-Nanoluc, miniPthl-tetR cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

PGus2A-TetO2/1

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJOE5751.1

Plasmid

#135083

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-His tag-eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHsp18.2 (GB0153)

Plasmid

#68171

Purpose

Provides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp18.2 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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FL-fibulin-3 pcDNA4

Plasmid

#29700

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pHsp70B (GB0155)

Plasmid

#68172

Purpose

Provides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtHsp70B promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1-3HA

Plasmid

#41610

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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