Addgene

We narrowed to 16,239 results for: multi

Showing: 4541 - 4560 of 16239 results

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pQCasTns(Vch)-entry(BsaI)

Plasmid

#190273

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(BsaI)

Use

Tags

Expression

Bacterial

Mutation

no

Promoter

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN380

Plasmid

#205076

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 380 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN296

Plasmid

#205075

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Lacks N Terminal 296 amino acids

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-yATOM_SH2-KDEL

Plasmid

#209714

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-yATOM_SH2-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5 (R80A)

Plasmid

#209713

Purpose

Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5 (R80A)

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

Plasmid

#209718

Purpose

Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-NbGFP_miRFP670nano3-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-Clover-KDEL

Plasmid

#209720

Purpose

Mammalian expression of clover localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-Clover-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Cox4-yATOM_SH2

Plasmid

#209715

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

Cox4-yATOM_SH2

Use

Tags

Cos4

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-blast

Plasmid

#208033

Purpose

Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoR

Plasmid

#193811

Purpose

CMV overexpression of ChemoR in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR

Use

Tags

10xHis

Expression

Mammalian

Mutation

mScarlet: D201K

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoC-CaM

Plasmid

#193813

Purpose

Protein production of ChemoC-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC-CaM

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoY-CaM

Plasmid

#193814

Purpose

Protein production of ChemoY-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoY-CaM

Use

Tags

10xHis

Expression

Bacterial

Mutation

Venus:A206K

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoR-CaM

Plasmid

#193815

Purpose

Protein production of ChemoR-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-CaM

Use

Tags

10xHis

Expression

Bacterial

Mutation

mScarlet: D201K

Promoter

T7

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1_CSL3

Plasmid

#200173

Purpose

Amino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

Depositor

Michael Jewett

Article

Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

Insert

CSL3

Use

Tags

6x His tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1_SUL-I

Plasmid

#200172

Purpose

Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vector

Depositor

Michael Jewett

Article

Warfel et al Glycobiology. 2023 Mar 7:cwad018. doi: 1

Insert

SUL-I

Use

Tags

6x His tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-258

Plasmid

#195438

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - pSFFV - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-259

Plasmid

#195439

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF3 BS - pSFFV - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-260

Plasmid

#195440

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF10 BS - pSFFV - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-322

Plasmid

#195441

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - minTK - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-323

Plasmid

#195442

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF3 BS - minTK - mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge