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pWL89A 2xE

Plasmid

#145792

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

2xE

Use

Tags

Expression

Yeast

Mutation

Insertion of 2 copies of the CMV Enhancer

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A 4xE

Plasmid

#145793

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

4XE

Use

Tags

Expression

Yeast

Mutation

Insertion of 4 copies of the CMV Enhancer

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV(shRNA)-Puro-6 CCEPR Vb2

Plasmid

#108278

Purpose

shRNA for CCEPR lncRNA

Depositor

Karl Munger

Article

Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

Insert

CCEPR (CCEPR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 R138S

Plasmid

#87659

Purpose

Flag/HA tagged HPV8 E6 R138S

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

R138S

Promoter

CMV

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K64A

Plasmid

#87656

Purpose

Flag/HA tagged HPV8 E6 K64A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

K64A

Promoter

CMV

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K136N

Plasmid

#87657

Purpose

Flag/HA tagged HPV8 E6 K136N

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

K136N

Promoter

CMV

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 K142A

Plasmid

#87660

Purpose

Flag/HA tagged HPV8 E6 K142A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

K142A

Promoter

CMV

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 L59D

Plasmid

#87655

Purpose

Flag/HA tagged HPV8 E6 L59D

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

L59D

Promoter

CMV

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV MmuPV1 E6 R130A

Plasmid

#87654

Purpose

Flag/HA tagged MmuPV1 E6 R130A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MmuPV1 E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

R130A

Promoter

CMV

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA1_pNeurog2-Luciferase reporter

Plasmid

#64160

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for pNeurog2-Luciferase reporter

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pAG250

Plasmid

#33204

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

NEK6 3'UTR (NEK6 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCpSRb-5

Plasmid

#13727

Depositor

Thomas Schwartz

Article

Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

Insert

Signal recognition particle receptor β subunit (SRP102 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

SRβ circularly permuted D210E188 improved solubil…

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pSPgRNA

Plasmid

#47108

Purpose

Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

SPgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al Elife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Use

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#133786

Purpose

Cre-dependent tdTomato fluorescent reporter for cell labeling

Depositor

Michael Stryker

Article

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

pAAV-EF1a-DIO-tdTomato-WPRE

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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HIV-dual-GT

Plasmid

#122696

Purpose

Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

Depositor

Klaus Überla

Article

Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

Inserts

mTagBFP2
DsRed-max

Use

Lentiviral

Tags

PEST domain

Expression

Mutation

Promoter

HIV-1 LTR

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Mutation

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMRE145

Plasmid

#118497

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-LAP2-mCherry

Plasmid

#159524

Purpose

Expresses mCherry from LAP2 promoter

Depositor

Esteban Engel

Article

Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

LAP2

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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