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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Mutation

Promoter

CBh

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Article

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

Plasmid

#193942

Purpose

a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

Depositor

Greg Delgoffe

Article

Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

Insert

heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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Plenti-CMV-MCS-RFP-SV-puro

Plasmid

#109377

Purpose

This plasmid expresses dsRed2 and puromycin resistance

Depositor

Behzad Gerami-Naini, Jonathan Garlick

Type

Empty backbone

Use

Lentiviral

Tags

dsRed2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER Long P2A

Plasmid

#164107

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Mt-ER Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-2

Plasmid

#218797

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-GCaMP3

Plasmid

#64853

Purpose

Cytosol-targeted version of the Ca2+ indicator GCaMP3.

Depositor

Jin Zhang

Article

Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

GCaMP3

Use

Tags

Nuclear export signal (NES)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has Service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG and GfaABC1D

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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