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Plasmid#144195PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertDMRTA2 (DMRTA2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.G469V
Plasmid#82833PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationG469VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.N581S
Plasmid#81687PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationN581SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp13_GC3opt
Plasmid#157714Purposemammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoterDepositorInsertNsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimized; gene expression was optimized by…PromoterAvailable sinceAug. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
CS2-Myc-hASUN
Plasmid#47955PurposeExpression of tagged-human ASUNDepositorInsertAsunder (INTS13 Human)
UseTagsMycExpressionMammalianMutationPromoterSp6Available sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNoxo1
Plasmid#58338Purposeexpresses mouse Noxo1 in mammalian cellsDepositorInsertNADPH oxidase organizer 1 (Noxo1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.G466V
Plasmid#81710PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationG466VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRAF_p.L597Q
Plasmid#81684PurposeGateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.DepositorInsertBRAF (BRAF Human)
UseGateway entry vectorTagsExpressionMutationL597QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BRR2 pRS313
Plasmid#111411PurposeBrr2 (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mito-mStayGold_pTwistCMVBetaGlobWPRENeo
Plasmid#221898PurposeExpresses mStayGold targeted to the mitochondriaDepositorInsertcox8-mStayGold
UseTagsCox8ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
p53 (dominant negative R175H mutant)-pcw107-V5
Plasmid#64638Purpose3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insertDepositorInsertTP53 (transcript variant 3) (TP53 Human)
UseLentiviralTagsV5ExpressionMutationR175HPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTon1(acgfp1)
Plasmid#233648PurposeExpression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInserttarget sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onTagsExpressionMutationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTon1(il11)
Plasmid#233647PurposeExpression vector of X. laevis il11.L-P2A-AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.DepositorInsertsinterleukin-11.L (350-913) (il11.L Frog)
target sequence of guide RNA #tyr (3905-3927) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
UseAmphibian expression, tet-onTagsExpressionMutationSome synonymous substitutions were introduced in…PromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
p324_DG
Plasmid#234690PurposeGolden gate domesticated pET28b with TEV-His6DepositorTypeEmpty backboneUseTagsTEV-His6ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p370_DG
Plasmid#234693PurposeGolden gate domesticated pET28b with 3C-mNeongreen-His10DepositorTypeEmpty backboneUseTags3C-mNeongreen-His10ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p277_DG
Plasmid#234695PurposeGolden gate domesticated pET28b for scarless cloning with His6-3CDepositorTypeEmpty backboneUseTagsHis6-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p357_DG
Plasmid#234700PurposeGolden gate domesticated pET28b for scarless cloning with His10-GST-3CDepositorTypeEmpty backboneUseTagsHis10-GST-3CExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
p368_DG
Plasmid#234691PurposeGolden gate domesticated pET28b with 3C-His6DepositorTypeEmpty backboneUseTags3C-His6ExpressionBacterialMutationNoPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only