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Plasmid#145658PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046730
Plasmid#145659PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046823
Plasmid#145634PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046816
Plasmid#145622PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046791
Plasmid#145586PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046774
Plasmid#145574PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A
Plasmid#127786PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32 and Arg36 to AlaPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R438A-K441A
Plasmid#127791PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg4…PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-TTM
Plasmid#127794PurposeExpresses the TTM/CYTH domain of ygiF in E. coli cellsDepositorInsertygiF (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 1-200)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S10
Plasmid#127829PurposeProduction of recombinant human ribosomal protein S10DepositorInsertRPS10 (RPS10 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF736
Plasmid#121893PurposeEF1a-dTEV-protease-GFPDepositorInsertsTEV protease
eGFP
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoPD
Plasmid#114466PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoPDDepositorInsertBoPD
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR161Q
Plasmid#92413PurposehQPRTase harboring R161Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR161QPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIAhqprtR161G
Plasmid#92412PurposehQPRTase harbouring R161G mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR161GPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102Q
Plasmid#92407PurposehQPRTase harboring R102Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102QPromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtK171S
Plasmid#92404PurposehQPRTase harbouring K171S mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationK171SPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR138Q
Plasmid#92411PurposehQPRTase harbouring R138Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR138QPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtK139A
Plasmid#92403PurposehQPRTase harbouring K139A mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV cleacable 6xHisExpressionBacterialMutationK139APromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only