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Plasmid#55328PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
hAAVS1 TALEN Right
Plasmid#52342PurposeTALEN vector for genomic targetingDepositorInsertAAVS1 TALEN Right
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-humanTRAF6 (1093)
Plasmid#66929PurposeMammalian Expression of TRAF6DepositorInsertTRAF6 (TRAF6 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42229PurposeThis plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T1
Plasmid#41817PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His(-)-HSulf-1
Plasmid#13003DepositorInserthuman sulfatase 1 (SULF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-NF1-GRD
Plasmid#19993DepositorInsertNeurofibromatosis type I (NF1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-ReaChR-citrine
Plasmid#50954PurposeReaChR-citrine in AAV2 vector under human synapsin promoterDepositorInsertReaChR-citrine
UseAAVTagscitrineExpressionMutationPromotertruncated human Synapsin promoterAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Archon1-KGC-EGFP-ER2-WPRE
Plasmid#108423PurposeArchon1 fluorescent voltage reporter in mammalian expression plasmidDepositorInsertArchon1-KGC-EGFP-ER2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG4
Plasmid#99578PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotypeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk2a1
Plasmid#13754DepositorInsertJnk2a1 (MAPK9 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf3b-IRES-Puro
Plasmid#141384PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf3b
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pME-ERT2-Cre-ERT2
Plasmid#82587PurposeMiddle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombinationDepositorInsertERT2-CRE-ERT2
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4-ERSE1-luc2P-Hygro
Plasmid#101789PurposeLuferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)DepositorInsert3xERSE-I
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCR4-Shh::lacZ-H11
Plasmid#139098PurposeenSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)DepositorTypeEmpty backboneUseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterShhAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNLS-iRFP670
Plasmid#45466DepositorInsertiRFP670
UseTagsnuclear localization signalExpressionMammalianMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-590
Plasmid#38314DepositorInsert5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only