Addgene

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Showing: 4501 - 4520 of 16227 results

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LI B-C N-NES KOZ b c7

Plasmid

#54445

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear Export Signal (NES) of heat stable inhibitor (PKI)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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GST-GW1D12(aa895-1211)

Plasmid

#21528

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contain aa895-1211

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
GST-GW1D1(aa254-751)

Plasmid

#21523

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contain aa254-751

Promoter

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only
pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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piHGpd/T101I

Plasmid

#1112

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 101 changed to isoleucine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'MS2-gRNA GG backbone (MOBE1-2)

Plasmid

#219948

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-3'end (S. pyogenes Cas9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-E46K

Plasmid

#181723

Purpose

Galactose inducible expression of Alpha-synuclein-E46K

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Use

Tags

Expression

Yeast

Mutation

E46K

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-A53T

Plasmid

#181722

Purpose

Galactose inducible expression of Alpha-synuclein-A53T

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Use

Tags

Expression

Yeast

Mutation

A53T

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-ATXN1-Q85

Plasmid

#181712

Purpose

Galactose inducible expression of ATXN1-Q85

Depositor

Alejandro Chavez

Insert

ATXN1-Q85 (ATXN1 Human)

Use

Tags

Expression

Yeast

Mutation

extended repeat of 85 Gln residues replacing nati…

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PtetA

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Ptac-VSVG

Plasmid

#218365

Purpose

Empty backbone for Tn7-site insertion with lacIq-Ptac expression system and C-terminal VSVG fusions

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Use

Tags

VSVG

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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nCas9-NG_P2A-mCherry

Plasmid

#219934

Purpose

Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL682

Plasmid

#205591

Purpose

PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL683

Plasmid

#205593

Purpose

Cas9-PE2 for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

ZmUbi

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS18

Plasmid

#218718

Purpose

Expresses N-terminally EGFP-tagged BBS18 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS18

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pWH1266-Apra-sgRNA

Plasmid

#218372

Purpose

sgRNA expression plasmid for CRISPR-intereference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

sgRNA expression cassette

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-FUS-R521C

Plasmid

#181718

Purpose

Galactose inducible expression of FUS-R521C

Depositor

Alejandro Chavez

Insert

FUS (FUS Human)

Use

Tags

Expression

Yeast

Mutation

R521C

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-FUS-P431L

Plasmid

#181717

Purpose

Galactose inducible expression of FUS-P431L

Depositor

Alejandro Chavez

Insert

FUS (FUS Human)

Use

Tags

Expression

Yeast

Mutation

P431L

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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