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pTC208

Plasmid

#70017

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBALU1

Plasmid

#69316

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfp

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

GFPint-FgF

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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Keratin 18 S33A

Plasmid

#18061

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Use

Tags

Expression

Mammalian

Mutation

S33A

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
MTK4b_004

Plasmid

#123843

Purpose

Encodes spacer sequence as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP_I392N-talin1 (1-2541)

Plasmid

#166114

Purpose

expresses talin-1-I392N in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

I392N-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/PH* (W495L) uGFP

Plasmid

#14564

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

PH* (W495L)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pCMB-TLEr

Plasmid

#61188

Purpose

Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB7A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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MTK3b_020

Plasmid

#123801

Purpose

Encodes synTF ZF binding domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SynTF

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_110

Plasmid

#91202

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-HT_RcCHAD

Plasmid

#127782

Purpose

Expresses RcCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

RcCHAD

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 Y3F/CR* (C529S) uGFP

Plasmid

#14560

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Y3F/CR* (C529S)

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
MTK2_017

Plasmid

#123709

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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iXFGFR1

Plasmid

#31257

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR1-ICD-Fv’Fv (fgfr1-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FaGH16A

Plasmid

#100910

Purpose

Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH16A

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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