Addgene

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pcDNA3.1-ADAM7 (S703N)

Plasmid

#31141

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S703N

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pDNA2.0-6H-TEV-KRAS G12D

Plasmid

#159439

Purpose

Expresses KRAS G12D in E.coli

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS G12D (KRAS Human)

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.S310F

Plasmid

#82854

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S310F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.D845A

Plasmid

#82905

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D845A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.V842I

Plasmid

#81722

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I655V; V842I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.R897Q

Plasmid

#81714

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I655V; R897Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.S418T

Plasmid

#82859

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S418T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCEF-FKBP12-Bla2

Plasmid

#190946

Purpose

Protein fragment complementation

Depositor

Jakob Reiser

Article

Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

Insert

C-terminal fragment of BLAK fused to FKBP12

Use

Tags

Expression

Mammalian

Mutation

Promoter

CEF

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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AP1muA-Halo-ALFA-polyA-G418 HR

Plasmid

#229681

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Halo, ALFA

Expression

Mutation

Promoter

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5_C12orf49-miniTurbo

Plasmid

#155112

Purpose

transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Use

Tags

3x Flag and miniTurbo

Expression

Mammalian

Mutation

Promoter

CMV/TO inducible promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

Plasmid

#231549

Purpose

Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

Depositor

Manos Mavrakis

Article

Martins et al bioRxiv 2024.04.26.591305

Insert

ACTB (ACTB Human)

Use

Tags

msfGFP (intramolecular)

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc msfGFPΔC10-beta-actin

Plasmid

#231548

Purpose

Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al bioRxiv 2024.04.26.591305

Insert

ACTB (ACTB Human)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-P81nmt1-3FLAG

Plasmid

#19350

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

hphMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-P41nmt1-3FLAG

Plasmid

#19349

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

hphMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-hphMX6-P3nmt1-3FLAG

Plasmid

#19348

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

hphMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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PKC gamma DN

Plasmid

#21239

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

K380R

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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PKC gamma WT

Plasmid

#21236

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
PKC epsilon WT

Plasmid

#21240

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
pICSL50009

Plasmid

#50309

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50007

Plasmid

#50308

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal FLAG tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge