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Plasmid#129654PurposeFor generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1DepositorInsert2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV(shRNA)-Puro-U6-CCEPR
Plasmid#108277PurposeshRNA for CCEPR lncRNADepositorInsertCCEPR (CCEPR Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGFP-P2A
Plasmid#80808Purposenuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2ADepositorInsertminimal cytomegalovirus immediate early enhancer/promoter
UseTagsExpressionMutationPromoterAvailable sinceNov. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
Plasmid#61441PurposeDox-inducible MyoD with mRNA tag for smFISHDepositorInsertMyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
Myog'-2A-mCherryNLS-PGK-Purodtk
Plasmid#69547PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…ExpressionMutationSilent V205V mutation in myogenin gene to prevent…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092686
Plasmid#153895PurposeBacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTRDepositorInsertfragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionBacterialMutationNearly native encoding;inactivating mutations in …PromoterPromoter | t7 consensus from NEB, G is +1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-CaMKIIa-C1C2-EYFP
Plasmid#35519DepositorInsertChR1-ChR2 Chimera
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb-HsfA2
Plasmid#161012PurposePP7-tagged reporter driven by the Arabidopsis HsfA2 promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
HSP70-pp7i-mCh-UPG
Plasmid#161011PurposeC-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7DepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCMV 16E6no* delPDZ
Plasmid#47678PurposePDZ-binding motif is deletedDepositorInsertHPV 16 E6no* (E6 HPV, Human)
UseTagsFLAG and HAExpressionMammalianMutationdel first 7aa and last 6aa, V42L*PromoterCMVAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorInsertsUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNoxo1
Plasmid#58338Purposeexpresses mouse Noxo1 in mammalian cellsDepositorInsertNADPH oxidase organizer 1 (Noxo1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
TOPO CMV
Plasmid#68454PurposeTOPO construct expressing CMV for generation of GMAP-compatible promoterDepositorInsertCytomegalovirus Immediate-Early Promoter
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNMHCII-C2-GFP
Plasmid#46043DepositorInsertnonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMV immediate earlyAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pJMP8823
Plasmid#220901PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8835
Plasmid#220902PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8921
Plasmid#220903PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP8923
Plasmid#220904PurposeMobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PG-dCas9-novoDepositorInsertdCas9-novo
UseCRISPRTags3xMycExpressionBacterialMutationD10A, H840APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-EEA1
Plasmid#222419PurposeTo express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertEEA1 (EEA1 Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only