-
Plasmid#141049PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF4-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-SpCas9-HF2-2A-EGFP-L2
Plasmid#141050PurposeMutisite Gateway mediated vector constructionDepositorInsertSpCas9-HF2-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-EGFP-PBL-R1
Plasmid#141018PurposeMutisite Gateway mediated vector constructionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-EGFP-3'short
Plasmid#129749PurposeGene targeting in marmoset cellsDepositorInsertACTB (ACTB C. jacchus (common marmost))
UseMarmoset targetingTagsEGFPExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR 1-55 eGFP SV40
Plasmid#132651PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 55 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjACTB-EGFP-5'short
Plasmid#129748PurposeGene targeting in marmoset cellsDepositorInsertACTB (ACTB C. jacchus (common marmost))
UseMarmoset targetingTagsEGFPExpressionMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.18_Ex_Ef1a-HA-EGFP-SV40-Zeo
Plasmid#128454Purposelentiviral expression vector with Ef1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, N-terminal HA tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176E
Plasmid#131203Purposemammalian expression, tet RDepositorInsertRIPK2 S176E (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationS176EPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_Y474F
Plasmid#131204Purposemammalian expression, tet RDepositorInsertRIPK2 Y474F (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationY474FPromoterpCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LC801: pMVP (L3-L2) eGFP + WPRE
Plasmid#121771PurposepMVP L3-L2 entry plasmid, contains eGFP + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest in lentivirus vectors.DepositorInserteGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO102: pMVP (R4-R3) eGFP open
Plasmid#121731PurposepMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-MCP-2yeGFP(405)
Plasmid#121419PurposeExpressing MCP-2yeGFPDepositorInsertCUP1p-MCP-2yeGFP
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP
Plasmid#121509PurposeExpresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).DepositorInsertsgFah.intron9
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IRES-EGFP-Flag-hRabin8
Plasmid#118073PurposeExpresses Flag-tagged human Rabin8 in mammalian cellsDepositorInserthuman Rabin8 (RAB3IP Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1 alphaAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_shRNA resistant nmPKA-Cα-mEGFP
Plasmid#114144PurposeThe G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.DepositorInsertmouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
UseTagsExpressionMammalianMutationG2A mutation and silent mutation to resist shRNA …PromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS_L4139P-eGFP-3HA
Plasmid#108404PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.DepositorInsertPKD1 (PKD1 Human)
UseTags3xHA and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4119-eGFP-3HA
Plasmid#108393PurposeExpress cytoplasmic tail mouse PC1 from amino acid 4119 to 4293DepositorInsertPkd1 (Pkd1 Mouse)
UseTagseGFP-3HAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only