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Showing: 9001 - 9040 of 148587 results

  1. SMN2E7-Fluc-Nluc-reporter

    Plasmid
    #221005
    Purpose
    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    Fluc-SMN2E7-STOP-Nluc
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221005-plasmid-map-sequence-id-437012

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  10. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    104907-plasmid-map-sequence-id-206042

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  17. pCMV-Neo-Bam

    Plasmid
    #16440
    Purpose
    Contains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.
    Depositor
    Bert Vogelstein
    Article
    Baker et al Science. 1990 Aug 24. 249(4971):912-5.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2008
    Availability
    Academic Institutions and Nonprofits only
    16440-plasmid-map-sequence-id-211955

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  19. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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  20. pRelA*

    Plasmid
    #175590
    Purpose
    Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Use
    Tags
    Fused with a flexible GS linker to mVenus
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175590-plasmid-map-sequence-id-348362

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  22. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177942-plasmid-map-sequence-id-348206

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  26. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Use
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133451-plasmid-map-sequence-id-267048

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  37. TFORF0985

    Plasmid
    #142249
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF4 (KLF4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    142249-plasmid-map-sequence-id-385602

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Showing: 9001 - 9040 of 148587 results