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Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD3_mCherry
Plasmid#187385PurposeTo chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRTDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-ZEB1 3'UTR
Plasmid#79601PurposeFUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.DepositorInsertd2GFP-Zeb1 3'UTR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACTN2-pEGFP
Plasmid#52669PurposeOverexpression of GFP-tagged α-actinin-2DepositorInsertactinin 2 (ACTN2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-T.2-ABE8e
Plasmid#185669PurposeExpresses eNme2-T.2-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-T.2
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-hPKM1
Plasmid#44241DepositorInsertPyruvate Kinase M1 (PKM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PPP2R2B
Plasmid#16181DepositorInsertPP2A regulatory subunit B, beta isoform (PPP2R2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42(Q61L)
Plasmid#12600DepositorInsertEGFP-Cdc42 (Q61L) (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61LPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-miRFP703
Plasmid#113097PurposeGeneral donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-miRFP703
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC1802_pmax-RBFOX1N-dCasRx-C
Plasmid#118635PurposeTransient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag YAP2 5SA
Plasmid#27371DepositorInsertYAP2 (YAP1 Human)
UseTagsflagExpressionMammalianMutationSerines at positions 61, 109, 127, 128, 131, 163,…PromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
12xMoonTag-12xSunTag-kif18b-24xPP7
Plasmid#128606PurposeExpresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon DREADD Gi-mCherry
Plasmid#177669PurposeExpresses DREADD Gi only in Flp expressing cells and not in Cre expressing cellsDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-3xFLAG
Plasmid#207695PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
Str-Golgin84_VSVG-SBP-EGFP
Plasmid#65305Purposesynchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TetOne-Puro-p62
Plasmid#204548PurposeExpresses p62 in a doxycycline-dependent manner in mammalian cellsDepositorInsertp62 (SQSTM1 Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpliceExpress
Plasmid#32485DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PH-Akt-GFP
Plasmid#51465PurposeIs a biosensor for PIP3/PI(3,4)P2DepositorInsertAKT (aa 1–164)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Hepatitis C Virus NS3/NS4A
Plasmid#61696Purposeinducible dual expression of NS3 protease along with its cofactor NS4ADepositorInsertsNS4A
NS3
UseTagshisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE2
Plasmid#73020PurposeExpresses BE2 in mammalian cellsDepositorInsertBE2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS SARS-CoV-2 BA.4/5 Spike
Plasmid#186031PurposeExpresses codon-optimized full length SARS-CoV-2 BA.4/5 SpikeDepositorInsertSARS-CoV-2 BA.4/5 Spike (S )
UseTagsExpressionMammalianMutationBA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…Promoterchicken β-actin promoter, CMV enhancerAvailable sinceJune 8, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-hPR-A
Plasmid#89119Purposemammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933DepositorInserthuman progesterone receptor-A (PGR Human)
UseTagsExpressionMammalianMutationhuman progesterone receptor-A, equivalent to huma…PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB3
Plasmid#120288PurposeProduce human recombinant TGFB3 in DE3 cellsDepositorInsertTransforming Growth Factor-β3 (TGFB3 Human, Synthetic)
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMCL1
Plasmid#178203PurposeAn improved vector for BacMam systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits