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Plasmid#51568PurposeConstitutively express bud neck localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCDC10 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNLuc_SV40:PuroR
Plasmid#183036PurposeExpresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KA1875_pGL4-UBCmin-luc2/Sox2_SRR2
Plasmid#124198PurposeLuciferase reporter driven by the Sox2 enhancerDepositorInsertSox2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0747 (pDonor_L-MmeI)
Plasmid#130647PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (L*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATG --> TGTTGGAGPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 1
Plasmid#36111DepositorInsertG protein beta subunit B1 (GNB1 Bovine)
UseTagsmcherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.4
Plasmid#158406PurposeExpress single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-G60A-FLAG
Plasmid#102323PurposeHuman SIRT6 G60A with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationchanged glycine 60 to alaninePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL0746 (pDonor_R-MmeI)
Plasmid#130646PurposeEncodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (R*)
UseCRISPR; TransposonTagsExpressionBacterialMutationTGTTGATA --> TGTTGGAAPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-RNF169
Plasmid#99392PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169DepositorInsertRNF169 (RNF169 Human)
UseTagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-NLS
Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Htb2
Plasmid#51569PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsHTB2 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HSC1-GiP
Plasmid#58254PurposeThis self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassetteDepositorInsertEnhanced Green Fluorescence Protein, Puromycin resistance gene
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceOct. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-eAFID-3
Plasmid#167360PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3Bctd-Cas9-UDG-T2A-AP lyase
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Spc72
Plasmid#51582PurposeConstitutively express spindle pole body localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSpc72 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1P-E4-luciferase
Plasmid#135592PurposeShort form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vectorDepositorInsertShort form promoter of human CYP26A1 gene (E4)
UseTagsExpressionMutationPromoterHuman CYP26A1 promoter (Short Form, E4)Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA1868_pGL4-UBCmin-luc2/Utf1-A'
Plasmid#124194PurposeLuciferase reporter driven by the Utf1 enhancerDepositorInsertUtf1 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-PEX3
Plasmid#51572PurposeConstitutively express peroxisome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsPEX3 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pH-AFID-3
Plasmid#167361PurposeFor inducing predictable deletions in rice plantsDepositorInsertA3A-Cas9-UDG-T2A-AP lyase
UseTagsExpressionPlantMutationWTPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScalps eSpCas9_Zsgreen gRNA scaffold
Plasmid#188449PurposeExpression of eSpCas9 tagged with ZsgreenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterEF1-alphaAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)TK2ALuc2/CMV/WPREdU3
Plasmid#163965PurposeCo-expression of HSV-1 TK and firefly luciferase in mammalian cellsDepositorInsertHSV-1 TK and FLuc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
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V5-WT-short-UBASH3A
Plasmid#192088PurposeMammalian expression of human wild-type short-form UBASH3A with V5 tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 3
Plasmid#36103DepositorInsertG protein subunit Gamma 3 (Gng3 Mouse)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pLLX13
Plasmid#79825PurposeYeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vectorDepositorTypeEmpty backboneUseYeast and bacteria shuttle vector, contains origi…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1870_pGL4-UBCmin-luc2/Esrrb-B'
Plasmid#124196PurposeLuciferase reporter driven by the Esrrb enhancerDepositorInsertEsrrb enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Claspin-Flag
Plasmid#136246PurposeProtein expression of Claspin with a C terminal FLAG tagDepositorInsertClaspin
UseTagsFlagExpressionMammalianMutationE263G, Q499R, I783T, D932G and S1062G- Please see…PromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/CGA-mEGFP-IRES-mCherry
Plasmid#49233PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
KA1869_pGL4-UBCmin-luc2/Fgf4-A'
Plasmid#124195PurposeLuciferase reporter driven by the Fgf4 enhancerDepositorInsertFgf4 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-dKeNLuc_SV40:PuroR
Plasmid#183046PurposeExpresses the FLAG-tagged dKeNLuc (Far-Red dKeima LumiFluor) in mammalian cells.DepositorInsertdKeNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-KaNLuc_SV40:PuroR
Plasmid#183047PurposeExpresses the FLAG-tagged KaNLuc (Far-Red LSSmKate2 LumiFluor) in mammalian cells.DepositorInsertKaNLuc LumiFluor
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-miR-148a
Plasmid#65052PurposeExpression of miR148a in mammalian cellsDepositorInserthsa-miR-148a (pre-miRNA) (MIR148A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU1/Sm/3' Box_(GLuc)_INT
Plasmid#68438PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pMC_mNG2(11)_BSDminus_F2
Plasmid#184164PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC_mNG2(11)_BSDminus_F1
Plasmid#184163PurposeDonor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmNG2(11)
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT2
Plasmid#118984PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationmutK2-SP-K1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT4
Plasmid#118986PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationK2-SP-mutK1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC-KIT6
Plasmid#118988PurposeMutational dissection of the G rich region of the c-kit promoterDepositorInsertc-kit proto-oncogene {promoter} (KIT Human)
UseTagsExpressionMammalianMutationmutK2-SP-mutK1PromoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 3
Plasmid#36113DepositorInsertG protein beta subunit B3 (GNB3 Human)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 4
Plasmid#36114DepositorInsertG protein beta subunit B4 (Gnb4 Mouse)
UseTagsmcherryExpressionMammalianMutationPromotercmvAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pCru5-/CGA-mEGFP-IRES-mCherry
Plasmid#49224PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
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KA1874_pGL4-UBCmin-luc2/Sox2_SRR1
Plasmid#124197PurposeLuciferase reporter driven by the Sox2 enhancerDepositorInsertSox2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA2627_pGL4-UBCmin-luc2/Jam2-A'
Plasmid#124200PurposeLuciferase reporter driven by the Jam2 enhancerDepositorInsertJam2 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2
Plasmid#124193PurposeLuciferase reporter driven by the Pou5f1 enhancerDepositorInsertPou5f1 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3
Plasmid#124192PurposeLuciferase reporter driven by the Pou5f1 enhancerDepositorInsertPou5f1 enhancer
UseLuciferaseTagsExpressionMutationPromoterUBCminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only