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pcDNA3-FBL6-FLAG3C

Plasmid

#19904

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

FBL6 (FBXL6 Human)

Use

Tags

FLAG3

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pWPI-E2F2

Plasmid

#114297

Purpose

Constitutive expression of the E2F2 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F2 (E2F2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 ΔC (1-381)

Plasmid

#62973

Purpose

Expresses HA-tagged rat SOX9 residues 1-383

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

codes for residues 1-381

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pICH67131

Plasmid

#153223

Purpose

Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, NPTII, Nos terminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pVRb01_syn20

Plasmid

#49704

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf01_syn20

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRedCm

Plasmid

#176225

Purpose

LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB and PL

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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XE118 CAMKII-T286D-CS2+

Plasmid

#16736

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-T286D (Camk2a Rat)

Use

Xenopus expression

Tags

Expression

Mutation

threonine 286 mutated to aspartate

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pHR RPS27A-3xFlag_IRES-GFP

Plasmid

#198390

Purpose

RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCFD4d-U6-1:white1-U6-3:white1

Plasmid

#84005

Purpose

pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

Depositor

Phillip Zamore

Article

Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

Inserts

white sgRNA-1
white sgRNA-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6:1, U6:3, and dU6-1; dU6-3

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 ∆24-mCherry

Plasmid

#80271

Purpose

mammalian expression plasmid for Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1A-BRD3R

Plasmid

#130696

Purpose

Lentiviral plasmids encoding human BRD3R

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

Human BRD3R (BRD3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 S15D

Plasmid

#69005

Purpose

mammalian expression of human p53 S15D mutant

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

S15D

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pFlox_N-Nipbl_BSD_FKBPF36V_EYFP

Plasmid

#163882

Purpose

Acute Depletion of NIPBL-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Nipbl (Nipbl Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pVRb20_992

Plasmid

#49714

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf20_992

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-1290

Plasmid

#78579

Purpose

Expression flag-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-1290 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutate 1291 residue to stop condon

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pSF-pMB1'-t7- YFP

Plasmid

#186564

Purpose

pMB1' ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-Archer1-EGFP

Plasmid

#60423

Purpose

Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

Depositor

Viviana Gradinaru

Article

Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

Insert

Archer1-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

Plasmid

#216380

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GST

Plasmid

#41611

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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