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Plasmid#75792Purpose3rd generation lentiviral gRNA plasmid targeting human SGK223DepositorInsertSGK223
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SGK223 gRNA (BRDN0001147947)
Plasmid#75793Purpose3rd generation lentiviral gRNA plasmid targeting human SGK223DepositorInsertSGK223
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADRBK1 gRNA (BRDN0001144871)
Plasmid#75681Purpose3rd generation lentiviral gRNA plasmid targeting human ADRBK1DepositorInsertADRBK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADRBK1 gRNA (BRDN0001145957)
Plasmid#75682Purpose3rd generation lentiviral gRNA plasmid targeting human ADRBK1DepositorInsertADRBK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADRBK1 gRNA (BRDN0001147663)
Plasmid#75683Purpose3rd generation lentiviral gRNA plasmid targeting human ADRBK1DepositorInsertADRBK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADRBK1 gRNA (BRDN0001147919)
Plasmid#75684Purpose3rd generation lentiviral gRNA plasmid targeting human ADRBK1DepositorInsertADRBK1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZAK gRNA (BRDN0001146874)
Plasmid#75598Purpose3rd generation lentiviral gRNA plasmid targeting human ZAKDepositorInsertZAK
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PRPF4B gRNA (BRDN0001146471)
Plasmid#76430Purpose3rd generation lentiviral gRNA plasmid targeting human PRPF4BDepositorInsertPRPF4B
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP
Plasmid#71666PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLS-EF1a-FRB-SpCas9-A
Plasmid#138477PurposeExpresses FRB fused SpCas9 for NanoMEDIC packaging.DepositorInsertSpCas9, human codon optimized
UseCRISPRTags3x HA tag and FRBExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_v2
Plasmid#74407PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)
Plasmid#71684PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-EGFP (ANV)
Plasmid#71685PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, Synthetic, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …PromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(+6)
Plasmid#89728PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a longer versi…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-6)
Plasmid#89730PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-12)
Plasmid#89731PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-18)
Plasmid#89732PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-3)
Plasmid#89729PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only