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Plasmid#224213PurposeBacteroides genomic editing tool; IPTG inducible-Fncpf1 and recTDepositorInsertFncpf1 and recT
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J
Plasmid#146690PurposeInsect Expression of DmHPat_57-968-dsRNAresDepositorInsertDmHPat_57-968-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation A1758G and one non silent G56…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J
Plasmid#146695PurposeInsect Expression of DmHPat_57-968-del500-684DepositorInsertDmHPat_57-968-del500-684 (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat_57-968-dsRNAres_H
Plasmid#146464PurposeInsect Expression of DmHPat_57-968-dsRNAresDepositorInsertDmHPat_57-968-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation T477C and one non silent muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP754_L2pV1_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192399PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.DepositorInsertAct2::FRT-Rluc-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP753_L2pV1_NOT_FRT_Rluc_0I_lacZ
Plasmid#192398PurposeOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its FRT sites.DepositorInsertAct2::FRT-Rluc-FRT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP733_L2_pV01_0I_FRT-uORF-rLUC_TCTP-fLUC
Plasmid#192376PurposeTo test for the repressive potential of the conserved peptide uORF from AT1G36730 with the FRT sites around it.DepositorInsertAct2::FRT-uORF-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP727_L2_pV01_0I_TMV-rLUC_TCTP-fLUC
Plasmid#192370PurposeTo control for variation introduced to the gene circuits, Rluc expression not affected by the introduction of terminators or recombination target sites.DepositorInsertAct2::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP732_L2_pV01_0I_FRT-35Ster-Rluc_lacZ
Plasmid#192375PurposeTo test for the repressive potential of the 35S terminator from CaMV with the FRT sites around it.DepositorInsertAct2::FRT-35Ster-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP731_L2_pV01_0I_FRT-OCStrunc-Rluc_lacZ
Plasmid#192374PurposeTo test for the repressive potential of the OCS variant (truncation) with the FRT sites around it.DepositorInsertAct2::FRT-OCStrunc-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP741_L2pV1_CR_B3RT-Rluc_TCTP-FlpO_lacZ
Plasmid#192385PurposeTo test for if Flp is able to de-repress a circuit with B3RT, to rule out cross-reactivity between these recombinases.DepositorInsertAct2::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP742_L2pV1_CR_FRT-Rluc_TCTP-B3o_lacZ
Plasmid#192386PurposeTo test for if B3 is able to de-repress a circuit with FRT, to rule out cross-reactivity between these recombinases.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP757_L2pV3_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192404PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP756_L2pV1_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192401PurposeOff state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.DepositorInsertAct2::B3RT-Rluc-B3RT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP755_L2pV1_NOT_B3RT_Rluc_0I_lacZ
Plasmid#192400PurposeOn state for the first generation of NOT gates, which target the Rluc CDS to repress expression via its B3RT sites.DepositorInsertAct2::B3RT-Rluc-B3RT
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ
Plasmid#192402PurposeOn state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ
Plasmid#192405PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its B3RT sites.DepositorInsertB3RT-Act2-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP713_L2pV3_NOT_FRT_Rluc_1IF_lacZ
Plasmid#192403PurposeOff state for the second generation of NOT gates, which target the Rluc promoter to repress expression via its FRT sites.DepositorInsertFRT-Act2-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ
Plasmid#192381PurposeTo test if the 35S promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP736_L2pV1_1I_Act2_FRT_OCS_TCTP_Flp_lacZ
Plasmid#192379PurposeTo test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output (despite the presence of the lacZ endlinker).DepositorInsertAct2::FRT-OCS-FRT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only