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Plasmid#145870PurposeInsect Expression of DmHPat_1-225DepositorInsertDmHPat_1-225 (Patr-1 Fly)
UseTagsExpressionInsectMutationone non silent mutation N218K compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_1-499_A
Plasmid#145871PurposeInsect Expression of DmHPat_1-499DepositorInsertDmHPat_1-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_226-499_A
Plasmid#145872PurposeInsect Expression of DmHPat_226-499DepositorInsertDmHPat_226-499 (Patr-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_500-968_A
Plasmid#145873PurposeInsect Expression of DmHPat_500-968DepositorInsertDmHPat_500-968 (Patr-1 Fly)
UseTagsExpressionInsectMutationone silent mutation A1758G compared to the sequen…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeIF4A-dsRNAres_AA
Plasmid#148201PurposeInsect Expression of DmeIF4A-dsRNAresDepositorInsertDmeIF4A-dsRNAres (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1-1596_O
Plasmid#147092PurposeMammalian Expression of HsXRN1_1-1596DepositorInsertHsXRN1_1-1596 (XRN1 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-ZZ-TEV-MZT2-3C-mEGFP
Plasmid#178074PurposeInsect cell expression of ZZ-TEV-MZT2-3C-mEGFPDepositorInsertMZT2A (MZT2A Human)
UseTagsPreScission-mEGFP and ZZ-TEVExpressionInsectMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-eGFP-KDEL
Plasmid#177727PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminusDepositorInserthFluc
UseTagsKDEL, Prolactin Signal Sequence, and eGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 Y635A
Plasmid#175772PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Y635ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationY635APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 N637A
Plasmid#175773PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 N637ADepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutationN637APromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN D474N
Plasmid#175762PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN D474NDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationD474NPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-EGFP-3'UTR#13
Plasmid#176338PurposeThe synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoterDepositorInsert3'UTR#13
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-30aa-Noxa-4E-pEGFP-C1
Plasmid#167529PurposeTo increase the length of the linker between Venus and Noxa-4E proteinsDepositorInsertPMA-induced protein 1 (PMAIP1 Human)
UseTagsVenusExpressionMammalianMutationC25E, L29E, F32E, L36EPromoterCMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-PML peptide
Plasmid#175247PurposeBacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycinDepositorInsertPML (PML Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR N52R (Shank3)
Plasmid#175255PurposeSPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with N52R mutation from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-13aa-Venus-pEGFP-C3
Plasmid#166753PurposeFor expression of mCerulean3 linked to Venus by 13 amino acid linkerDepositorInsertmCerulean3-13aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-79aa-ActA-pEGFP-C1
Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Synthetic, Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-BimEL(Noxa)-pEGFP-C1
Plasmid#166761PurposeTo examine effect of swapping the BH3 region in full length BimEL with that of NoxaDepositorUseTagsVenusExpressionMammalianMutationBH3 region removed and inserted BH3 of human Noxa…PromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-39aa-Venus-pEGFP-C3
Plasmid#166755PurposeFor expression of mCerulean3 linked to Venus by 39 amino acid linkerDepositorInsertmCerulean3-39aa-Venus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO::EGFP-hMeikin(8A mut)
Plasmid#174707PurposeDoxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 bindingDepositorInsertMeikin
UseTagsEGFPExpressionMammalianMutationS175A, T176A, T180A, S181A, S196A, T251A, T264A, …PromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only