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pSECC-sgSIK2-A

Plasmid

#138670

Purpose

Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 mouse (Sik2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK3-C

Plasmid

#138674

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK1-D

Plasmid

#138668

Purpose

Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK2-B

Plasmid

#138671

Purpose

Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 mouse (Sik2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECC-sgSIK1-E

Plasmid

#138669

Purpose

Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECC-sgSIK3-D

Plasmid

#138675

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Puro-sgSNRK

Plasmid

#138694

Purpose

Expresses a human SNRK-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSNRK human (SNRK Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Use

Tags

Expression

Bacterial

Mutation

Promoter

synthetic constitutive (stationary phase) promote…

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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ARB366

Plasmid

#124049

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to KRAB domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::KRAB-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB367

Plasmid

#124050

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFL I3

Plasmid

#113514

Purpose

Expression of I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-I3 V41A / W43A

Plasmid

#113515

Purpose

Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

V41A /W43A

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37delta2-81

Plasmid

#113502

Purpose

Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 2-81

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFL His-I3

Plasmid

#113513

Purpose

Expression of His-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154A/H250Y

Plasmid

#108491

Purpose

expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-INCENP d543-746

Plasmid

#108473

Purpose

expression of vsv-INCENP d543-746

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

INCENP d543-746 AA (INCENP Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Delta Alpha helix, aa543-746 deleted

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-AuroraB L154A/H250Y

Plasmid

#108489

Purpose

expression of vsv-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-623

Plasmid

#92266

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 6W6A (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

The following point mutations were introduced W23…

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-695

Plasmid

#92263

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

Depositor

Witold Filipowicz, Marina Chekulaeva

Article

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 PAM2 mut (gw Fly)

Use

Tags

NHA

Expression

Insect

Mutation

The following point mutations were introduced: EF…

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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