Addgene

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pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pXcX-PRS-EGFP

Plasmid

#25865

Depositor

Sergey Kasparov

Article

Teschemacher et al Hypertension. 2008 Aug . 52(2):351-8.

Insert

PRS driven enhanced green fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoEmerald-Kan

Plasmid

#44946

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEmerald

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133141

Plasmid

#152404

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133151

Plasmid

#153581

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-link-yomWasabi-SpHis5

Plasmid

#44850

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mWasabi with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomWasabi

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pVS45

Plasmid

#20059

Depositor

Stephen Sligar

Article

Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

Insert

rat hepatic cytochrome b5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRD134

Plasmid

#132378

Purpose

contains the sequence of mScarlet for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mScarlet

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046640

Plasmid

#153906

Purpose

Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CFP-CitA6-130-Venus

Plasmid

#128016

Purpose

Unmodified FLIP CitA6-130 sensor

Depositor

Uwe Sauer

Article

Ewald et al PLoS One. 2011;6(12):e28245. doi: 10.137

Insert

CitA

Use

Tags

Expression

Mutation

see Depositor Comments

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGD165

Plasmid

#132370

Purpose

contains the sequence of eGFP for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-eGFP

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pRD53

Plasmid

#132377

Purpose

contains the sequence of mCherry for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mCherry

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Rac1

Plasmid

#163607

Purpose

Expresses Rac1 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Rac1 (Rac1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133341

Plasmid

#152285

Purpose

Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGD255

Plasmid

#132376

Purpose

contains the sequence of Venus for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-Venus

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-spo5DSR

Plasmid

#48983

Purpose

for Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046406

Plasmid

#153880

Purpose

Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSth_Cas6

Plasmid

#178779

Purpose

Expression of S. thermophilus type I-E cas6

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

S. thermophilus type I-E cas6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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