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Showing: 11101 - 11150 of 156588 results

  4. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  11. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    David Sibley
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86664-plasmid-map-sequence-id-193761

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  12. TFORF0239

    Plasmid
    #142385
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MYCL (MYCL Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    142385-plasmid-map-sequence-id-391445

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  14. pcDNA3-TLR3-CFP

    Plasmid
    #13641
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 3 (TLR3 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13641-plasmid-map-sequence-id-7719

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  19. TSHR-Tango

    Plasmid
    #66518
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    TSHR (TSHR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66518-plasmid-map-sequence-id-343882

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  25. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  27. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid
    #226105
    Purpose
    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    atf4 (ATF4 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains uORF1-2 of ATF4, whose stability can ser…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226105-plasmid-map-sequence-id-448414

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  29. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid
    #160629
    Purpose
    TU for the expression of LbCas12a
    Depositor
    Diego Orzaez
    Insert
    AtUBQ10-:LbCpf1(Cas12a):Tnos
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    UBQ10
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160629-plasmid-map-sequence-id-321990

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  38. pDV002

    Plasmid
    #191823
    Purpose
    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR
    Depositor
    Jim Roberts
    Article
    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191823-plasmid-map-sequence-id-375876

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  39. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

    Plasmid
    #208956
    Purpose
    A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208956-plasmid-map-sequence-id-415745

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  43. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid
    #52092
    Purpose
    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP
    Depositor
    Dario Vignali
    Article
    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:
    Inserts
    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    Use
    Retroviral
    Tags
    F2A, IRES-EGFP, P2A, and T2A
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    52092-plasmid-map-sequence-id-80506

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  48. Cx36-GCaMP

    Plasmid
    #123604
    Purpose
    Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells
    Depositor
    John O'Brien
    Article
    Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19
    Insert
    Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
    Use
    Tags
    GCaMP3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    123604-plasmid-map-sequence-id-243949

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Showing: 11101 - 11150 of 156588 results