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Plasmid#50016PurposeMammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.DepositorInsertRanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
UseTags3 x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHTN_HaloTag_KIF5B
Plasmid#213404PurposeFull-length human kinesin-1 with HaloTag at the N-terminusDepositorInsertHaloTag-KIF5B (KIF5B Human)
UseTagsHaloTagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV1 NSP3 C-V5-tag
Plasmid#179962PurposeMammalian expression vector for SARS-CoV-1 Nsp3, V5-tagged. Sequence codon-optimized.DepositorInsertSARS-CoV-1 Nsp3 (ORF1ab Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Dvl2-GFP
Plasmid#109148PurposeStable Dvl2 expressionDepositorInsertDvl2 (DVL2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterSV40Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Brn2
Plasmid#27151DepositorInsertBrn2 (Pou3f2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
AbVec2.0-IGHG3
Plasmid#99577PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotypeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
hKv2.1-pEGFP-N1
Plasmid#111532PurposeExpresses human KCNB1 in mammalian cells for imagingDepositorInserthuman Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NUP50-N-10
Plasmid#55111PurposeLocalization: Nuclear Pore, Excitation: 587, Emission: 610DepositorInsertNUP50 (NUP50 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ104 - Pmyo-3::mCherry::unc-54
Plasmid#19328DepositorArticleInsertPmyo-3::mCherry
UseTagsmCherryExpressionWormMutationPromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Con/Fon DREADD Gq-mCherry
Plasmid#183532PurposeINTRSECT/ChemogeneticsDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG RpH-LAMP1-3xFLAG
Plasmid#163018Purposeexpresses ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EBFP2-nuc
Plasmid#36085DepositorTypeEmpty backboneUseLentiviralTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…PromoterAvailable sinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLPCX cyto Grx1-roGFP2
Plasmid#64975PurposeMammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)DepositorInsertGlutaredoxin-1 (GLRX Human)
UseRetroviralTagsroGFP2ExpressionMammalianMutationPromoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/cMG.WPP
Plasmid#184542PurposeAAV packaging plasmid, expressing Rep2 and AAV-cMG.WPP capsidDepositorInsertAAV-cMG.WPP capsid
UseAAV; Helper vector for packagingTagsExpressionMutationchanges on AAV9 capsid: K449R; insertion of WPPKT…PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294
Plasmid#186984Purposemammalian expression of cas7-11-r978-gggs-r1294DepositorInsertcas7-11-r978-gggs-r1294
UseTagsExpressionMammalianMutationr978-gggs-r1294 deletionPromoterAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV FST-FLAG
Plasmid#175167PurposeLentiviral expression of human FST-FLAGDepositorInsertFST (FST Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Cdc42-Q61L
Plasmid#12974DepositorInsertCdc42 constitutively active (CDC42 Human)
UseTagsMycExpressionMammalianMutationQ61L Constitutively activePromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
CMV-tdTomato-P2A-paCaMKII
Plasmid#165431PurposeExpresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.DepositorInserttdTomato-P2A-paCaMKII
UseTagstdTomatoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCDH-WT-ALKBH5-3xFLAG
Plasmid#203736PurposeOverexpression of ALKBH5-WTDepositorInsertALKBH5 (ALKBH5 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-Mito-7
Plasmid#57773PurposeLocalization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertMito (COX8A Human)
UseTagsminiSOGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAP114
Plasmid#120799PurposeE. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)DepositorInsertPxyl::mCherryOpt
UseTagsExpressionBacterialMutation1.4 kb xylR-Pxyl DNA fragment from C. difficile R…PromoterPxylAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_ATG4B_BamHI_NotI
Plasmid#190862PurposePlasmid for the expression and purification of of ATG4B. Internal reference: SMC1392DepositorInsertATG4B (ATG4B Human)
UseTagsGST, Thrombin Cleavage siteExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0513
Plasmid#141625PurposeLentiviral vector for overexpressing the PAX5 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPAX5 (PAX5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP13
Plasmid#22568DepositorInsertUSP13 (USP13 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag
Plasmid#209724PurposeExpresses cytosolic low affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95A.A119L.HaloTag
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi-tRNA
Plasmid#158578PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYS2_crtYf
Plasmid#85544PurposeConstitutive transcription of crRNA of crtYf, Spectinomycin resistanceDepositorInsertcrRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-sgBrca2#1/Cre
Plasmid#173627PurposeExpresses a Brca2-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Brca2 (Brca2 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-GFP
Plasmid#181906PurposeFluorescently tagged dead Cas9DepositorInsertdCas9
UseCRISPRTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDK1-FLAG-LOV*
Plasmid#202205PurposeExpresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cellsDepositorInsert[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)
UseTagsFLAG and LOV*ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC
Plasmid#99641PurposeBacterial expression of wildtype Rag GTPase dimerDepositorUseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tandem p40p35
Plasmid#108665PurposeExpression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linkerDepositorUseTagsExpressionMammalianMutationIL-12 dimers have a Gly-Ser linkerPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-H2BGFP
Plasmid#26790DepositorInsertH2B-GFP (H2BC21 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
ECFP-H2B-6
Plasmid#55344PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorInsertH2B (H2BC11 Human)
UseTagsECFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceOct. 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL2 co
Plasmid#90513PurposeLentiviral vector for expression of human codon optimized IL2 with puromycin resistance.DepositorInsertIL2 (IL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PDHA1-N-10
Plasmid#55118PurposeLocalization: Mitochondria, Excitation: 587, Emission: 610DepositorInsertPDHA1 (PDHA1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
mPlum-pBAD
Plasmid#54564PurposeLocalization: Protein Expression Vector , Excitation: 590, Emission: 649DepositorTypeEmpty backboneUseTags6xHis and mPlumExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDN34-BiNLS-hYAP1-1delta
Plasmid#169471Purposeexpresses optoYAP in mammalian cellsDepositorInsertYAP1-1delta (YAP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-mCherry
Plasmid#114469PurposemCherry ControlDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nanoHER2-SpyTag003
Plasmid#216312PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.DepositorInsertnanoHER2-SpyTag003
UseTagsHis6 tag and SpyTag003ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFRT_TO_eGFP_TIAL1
Plasmid#106091Purposegene expression of EGFP-TIAL1DepositorInsertTIAL1 (TIAL1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro
Plasmid#141390PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 8, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
hNET pcDNA3
Plasmid#15475DepositorInserthNET (SLC6A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only