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Flag-M4-AR
Plasmid#171240PurposeMammalian expression of 3xFlag-tagged human androgen receptor (hAR)DepositorInsert3xFlag-tagged human androgen receptor (AR Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA
Plasmid#12522PurposeRetroviral expression of HA tagged phosphoinositide 3-kinaseDepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
E7 in pENTR1A (w186-1)
Plasmid#22254DepositorInsertHuman papilloma virus E7 (E7 )
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmStrawberry-Atg4BC74A
Plasmid#21076DepositorInsertAtg4B(C74A) (Atg4b Mouse)
UseTagsmStrawberryExpressionMammalianMutation220-222 TGC(Cys) is changed to GCA (Ala)PromoterAvailable sinceJune 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
UseTags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Fon TVA-mCherry
Plasmid#131779PurposeINTRSECT 2.0DepositorInsertTVA-mCherry
UseAAV; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-p23
Plasmid#108224PurposeMammalian expression vector for mCherry fusion to human p23DepositorInsertmCherry-p23 (PTGES3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-3xFLAG-GFP-puroR
Plasmid#167463Purpose3rd-gen lentiviral expression of FLAG-GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab3P::his-24::mNeptune
Plasmid#124350PurposeNuclear Localized Pan-Neuronal mNeptuneDepositorInsertrab-3, mNeptune (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy56
Plasmid#122008PurposeMammalian vector for parallel SLIC cloning containing N-terminal TwinStrep and PreScission Cleavage siteDepositorTypeEmpty backboneUseTagsHis10/none and TwinStrepExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLPIT Cry2PHR-mCh-RhoA
Plasmid#42959DepositorInsertCry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
UseRetroviralTagsExpressionMammalianMutationPromoterTet-Off promoterAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
TKTL1
Plasmid#72419PurposeStudy the role of aberrant expression of TKTL1 in HNSCC tumorigenesisDepositorInsertTKTL1 (TKTL1 Human)
UseTagsExpressionMammalianMutationmay have one or two synonymous substitutionsPromoterCMVAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSCM167.2-Venus-VF-hADAM10(aa1-213)
Plasmid#189554PurposeProtein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-213PromoterAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hADAM10(aa220-654)
Plasmid#189560PurposeProtein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 220-654PromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCM167-Venus-VF-hADAM10(aa1-459)
Plasmid#189559PurposeProtein expression ADAM10 in mammalian cells. Contains prodomain and metalloprotease domain--amino acids 1-459DepositorInserttruncated ADAM metallopeptidase domain 10 (ADAM10 Human)
UseTagsVFExpressionMammalianMutationcontains aa 1-459PromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-IL6ST-COMP5AP-AviTag-9xHis
Plasmid#157485PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertIL6ST (IL6ST Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorInsertSnap-Omp25
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AC EJ7-GFP
Plasmid#113617PurposeReporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFPDepositorInsertEJ7-GFP variant of EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS2422_Nme2.Smu-ABE8e-i1
Plasmid#201520PurposeMammalian expression of domain inlaid Nme2.Smu-ABE8e-i1DepositorInsertNme2.Smu-ABE8e-i1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJZC603
Plasmid#62317PurposesgRNA with 2x PP7 for yeast cellsDepositorInsertsgRNA + 2x PP7 RNA binding module
UseTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGK:HygroR-CMV:mTagBFP2-A_UTR
Plasmid#68479PurposeLentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistanceDepositorInsertsHygromycin Resistance
mTagBFP2-A_UTR
UseLentiviralTagsExpressionMammalianMutationshRNA sensor cassette "A" added to 3&#x…PromoterCMV and pGKAvailable sinceNov. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-TRIM28 ts3
Plasmid#115864PurposeTRIM28 knockdownDepositorInsertTRIM28 shRNA (TRIM28 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0501 SFFV-mCherry-SGc(MYC.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191185PurposeExpression of MYC sensor in mammalian cellsDepositorInsertmCherry:MYC.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330.puro
Plasmid#110403PurposeCRISPR/Cas9 vector with sgRNA expression and puromycin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSGV Mouse Acceptor
Plasmid#128176PurposeA TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PIK3C2G
Plasmid#171946PurposeExpresses PIK3C2G in mammalian cellsDepositorInsertPIK3C2G (PIK3C2G Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLCO1A2_STOP
Plasmid#161342PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLCO1A2 (SLCO1A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
nls-mCherry
Plasmid#108881PurposeExpression of nuclear localized mCherryDepositorInsertnls-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only
