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pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp7-His

Plasmid

#11825

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 7 (CASP7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
CMV::SypHy A4

Plasmid

#24478

Depositor

Leon Lagnado

Article

Granseth et al Neuron. 2006 Sep 21. 51(6):773-86.

Insert

SypHy (Syp Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCI-neo-cOVA

Plasmid

#25097

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

cytoplasmic ovalbumin

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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HA-EGFP-HaloTag2

Plasmid

#41742

Depositor

Craig Crews

Article

Neklesa et al Nat Chem Biol. 2011 Jul 3;7(8):538-43. d

Insert

Halotag 2

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-ARP2-N-14

Plasmid

#54980

Purpose

Localization: Actin Related Protein, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ARP2 (ACTR2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pAG425GPD-ccdB

Plasmid

#14154

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1

Plasmid

#13676

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 (SMURF1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-CWB-cyan pre-eGRASP(p32)

Plasmid

#111579

Purpose

An AAV vector that expresses cyan pre-eGRASP(p32) under the CaMKIIa promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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Dynactin-4/p62-GFP

Plasmid

#31071

Depositor

Vann Bennett

Insert

Dynactin-4 (DCTN4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Utr230-EGFP-3XNLS

Plasmid

#58466

Purpose

Expresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

Inserts

Utr230 with 3X glycine linker (UTRN Human)
3X NLS with 3X stop codons

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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eIF2a 3

Plasmid

#21809

Depositor

David Ron

Insert

eIF2a (EIF2S1 Human)

Use

Tags

Expression

Mammalian

Mutation

S52D

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Use

Tags

T2A peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-sox2

Plasmid

#20326

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1(wt)

Plasmid

#13719

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(wt) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pUK21

Plasmid

#49788

Purpose

E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

lacZ

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_DA2h

Plasmid

#140554

Purpose

Expresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2h (high affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nMagHigh1-EGFP-CAAX

Plasmid

#67300

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

nMagHigh1-EGFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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