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Showing: 4421 - 4440 of 8438 results

  3. AICSDP-144:CTCF-mEGFP

    Plasmid
    #168799
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF
    Depositor
    Allen Institute for Cell Science
    Insert
    CTCF (CTCF Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168799-plasmid-map-sequence-id-334694

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  5. pJW1354

    Plasmid
    #69490
    Purpose
    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
    Depositor
    Jordan Ward
    Article
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    Use
    CRISPR; Template to generate cassettes for -media…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69490-plasmid-map-sequence-id-122661

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  7. pES134

    Plasmid
    #127551
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Article
    Wu et al Microbiology (Reading). 2024 Mar;170(3).
    Insert
    F3-flanked spectinomycin resistance cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127551-plasmid-map-sequence-id-251734

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  8. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

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  16. pSLC-239

    Plasmid
    #73193
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-yhaV (yhaV )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB
    Available since
    July 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73193-plasmid-map-sequence-id-134708

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  18. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid
    #133340
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133340-plasmid-map-sequence-id-269542

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Showing: 4421 - 4440 of 8438 results