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pQA-LP579

Plasmid

#222958

Purpose

Expresses R36S K51S LoaP double mutant

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 36 to Serine and Lysine 51 to Se…

Promoter

T7

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT203

Plasmid

#204512

Purpose

Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

C04F12.1 (C. elegans)

Use

Tags

6xHis, MBP

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHIT517

Plasmid

#204518

Purpose

Bacterial expression of CEC-4 with 6xHis and maltose binding protein tags for in vitro assays

Depositor

Yuji Kohara

Article

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

cec-4 (C. elegans)

Use

Tags

6xHis, MBP

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Use

Tags

6His-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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Atg32 [200-341] 1B

Plasmid

#190431

Purpose

Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

Depositor

Michael Ragusa

Article

Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

Insert

Atg32

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLz54_polyPro_polar

Plasmid

#140847

Purpose

Same as pLz47 but with 20 polar amino acids instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with polar residues

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz58_polyPro_CsoS2_CTP

Plasmid

#140850

Purpose

Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 C-terminal peptide

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz33_CsoS2_NTD_GFP

Plasmid

#140861

Purpose

His-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFP

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD fused to GFP

Use

Tags

Hexahistidine tag and Superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz55_polyPro_random_Npeptide

Plasmid

#140846

Purpose

Same as pLz47 but with randomized N*-peptide sequence

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with randomized CsoS2 N-peptide

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz56_polyPro_CsoS2_MR_r1

Plasmid

#140848

Purpose

Same as pLz47 but with MR repeat 1 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 MR repeat 1

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz57_polyPro_CsoS2_CTD_r8

Plasmid

#140849

Purpose

Same as pLz47 but with CTD repeat 8 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 CTD repeat 8

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Use

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Use

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pZF-AA-GCN

Plasmid

#127479

Purpose

coding sequence for synthetic zinc finger ZF-AA-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-AA fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Expression

Mutation

Promoter

T7

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPD1cR

Plasmid

#86970

Purpose

Expresses PCNA1 variant (G108C) fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA1 variant (G108C) fused to phosphite dehydrogenase and putidaredoxin reductase

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, G108C in PCNA1

Promoter

T7 promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pPD1R

Plasmid

#86969

Purpose

Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH

Promoter

T7 promoter

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pPD2cX

Plasmid

#86968

Purpose

Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

Insert

PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…

Promoter

T7 promoter

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-3

Plasmid

#85094

Purpose

Host vector to fuse a gene at the N-terminus of PCNA3

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 3

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pC3

Plasmid

#85087

Purpose

Expresses PCNA3 protein fused to P450cam (CYP101A1) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

PCNA3 fused to P450cam

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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