-
Plasmid#87678PurposeFlag/HA tagged MmuPV1 E6 R52ADepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationR52APromoterCMVAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiN MmuPV1 E6
Plasmid#87661PurposeLentiviral expression of MmuPV1 E6DepositorInsertMmuPV1 E6
UseLentiviralTagsFlag and HAExpressionMutationPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCMV MmuPV1 E6
Plasmid#87653PurposeFlag/HA tagged MmuPV1 E6DepositorInsertMmuPV1 E6
UseTagsFlag and HAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4A03
Plasmid#71027PurposeGateway entry cloneDepositorInsertlds (lds Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK E7 del EDE
Plasmid#13675DepositorInsertHPV 16 E7 del EDE (E7 Human)
UseTagsGSTExpressionBacterialMutationdel aa 35-37PromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCpSRb-4
Plasmid#13726DepositorInsertSignal recognition particle receptor β subunit (SRP102 Budding Yeast)
UseTags6xHisExpressionBacterialMutationCircularly permuted SRβD210K183 with Precission p…PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RosaR4 KKR mutations
Plasmid#37199DepositorInsertFokI (fused to RosaR4 ZFN)
UseZfn expressionTagsHA, ROSA26 eZFNs R4, and SV40 NLSExpressionMammalianMutationI538K, E490K, and H537RPromoterCMVAvailable sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-WT human MyoD-T2A-dsRedExpress2
Plasmid#60628PurposeExpresses WT human MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
cytoplasmic NAD+ biosensor
Plasmid#186787PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasmDepositorInsertcytoplasmic NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuclear NAD+ biosensor
Plasmid#186789PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleusDepositorInsertnuclear NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pLenti-mCherry-CAAX
Plasmid#129285PurposeLentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequenceDepositorInsertFluorescent reporter mCherry fused to CAAX polybasic sequence
UseLentiviralTagsCAAXExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
APEX2-OMM
Plasmid#79056PurposeSoybean APEX2 anchored to cytosolic face of outer mitochondrial membrane with C-terminal targeting sequence of last 31aa of human mitochondrial antiviral-signaling proteinDepositorInsertSoybean APEX2
UseLentiviralTagsflag and last 31 amino acids of human MAVSExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPIDNB
Plasmid#169901PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTSDepositorTypeEmpty backboneUseAivian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-mCherry
Plasmid#20938PurposeMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only