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pDON223 Spike H655Y

Plasmid

#180059

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike H655Y (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike H655Y

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike S982A

Plasmid

#180067

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike S982A (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike S982A

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike T1027I

Plasmid

#180058

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike T1027I (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike T1027I

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDON223 Spike D1118H

Plasmid

#180068

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D1118H (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D1118H

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDON223 Spike K417N

Plasmid

#180060

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike K417N (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike K417N

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDON223 Spike A701V

Plasmid

#180062

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A701V (S SARS-CoV-2)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A701V

Promoter

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pIRESneo2 mycBioID-Tollip M240A,F241A

Plasmid

#178055

Purpose

myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

Tags

mycBioID

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-U3-UL8

Plasmid

#166685

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA (UL29, UL8 HSV-1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC-TMPRSS11B

Plasmid

#172421

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 11B (TMPRSS11B Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MAC-IFITM3

Plasmid

#172419

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Interferon-induced transmembrane protein 3 (IFITM3 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET15b p97-His D592Amb

Plasmid

#169022

Purpose

Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His

Expression

Bacterial

Mutation

D592TAG

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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shFGF2#1

Plasmid

#157667

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shCdc20

Plasmid

#160954

Purpose

Cdc20 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdc20 shRNA (Cdc20 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shKif11

Plasmid

#160962

Purpose

Kif11 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Kif11 shRNA (Kif11 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shAspm

Plasmid

#160947

Purpose

Aspm shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Aspm shRNA (Aspm Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shCcnb2

Plasmid

#162794

Purpose

Ccnb2 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Ccnb2 shRNA (Ccnb2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMKO.1-shCenpf

Plasmid

#160956

Purpose

Cenpf shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cenpf shRNA (Cenpf Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSECC-sgSIK3-A

Plasmid

#138672

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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