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Showing: 4421 - 4440 of 150501 results

  1. TFORF2679

    Plasmid
    #144143
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PEG3 (PEG3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144143-plasmid-map-sequence-id-396223

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  2. pGL3basic luciferase and renilla_polyA

    Plasmid
    #128046
    Purpose
    pGL3 basic luciferase reporter with co-expression of renilla luciferase
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128046-plasmid-map-sequence-id-254873

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  3. pUCDM

    Plasmid
    #110742
    Purpose
    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site
    Depositor
    Tim Richmond
    Article
    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin and p10 promoters
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110742-plasmid-map-sequence-id-213591

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  5. HTR2A-Tango

    Plasmid
    #66409
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR2A (HTR2A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66409-plasmid-map-sequence-id-244394

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  6. pAAV-hSyn-eNpHR 3.0-EYFP

    Plasmid
    #26972
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by humanized Synapsin promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV5
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Trafficking Signal (TS) ER Export Signal
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26972-plasmid-map-sequence-id-196842

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  7. peSpCas9(1.1)-2×sgRNA (empty, donor)

    Plasmid
    #80768
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.
    Depositor
    Kazuhisa Nakayama
    Article
    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    80768-plasmid-map-sequence-id-171038

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  11. TFORF3228

    Plasmid
    #144704
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MAX (MAX Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144704-plasmid-map-sequence-id-404307

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  12. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid
    #208963
    Purpose
    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    EcKlenow-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208963-plasmid-map-sequence-id-415798

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  14. TFORF0801

    Plasmid
    #141707
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF3 (ATF3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141707-plasmid-map-sequence-id-391487

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  17. pOPINE GFP nanobody:Halo:His6

    Plasmid
    #111090
    Purpose
    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.
    Depositor
    Lennart Wirthmueller
    Article
    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:
    Insert
    GFP Nanobody
    Use
    Tags
    Halo and His6
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    T7 promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111090-plasmid-map-sequence-id-264731

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Showing: 4421 - 4440 of 150501 results