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pDONR223_KLRC2_p.G174R

Plasmid

#81270

Purpose

Gateway Donor vector containing KLRC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KLRC2 (KLRC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

N2S, F102S, G174R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEN12A-P1

Plasmid

#27366

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

Pmyc1tetO

Use

Gateway donor vector

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P41nmt1-3FLAG

Plasmid

#19340

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

his3 (his3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pOSV801

Plasmid

#126044

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Article

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P3nmt1-3FLAG

Plasmid

#19339

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

his3 (his3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P81nmt1-3FLAG

Plasmid

#19341

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Aug 26. ():.

Insert

his3 (his3 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pSLQ7501 pHR (hU6-crNT-EFS-PuroR-WPRE)

Plasmid

#214876

Purpose

Lentiviral vector encoding RfxCas13d nontargeting control guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crNT-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

Plasmid

#214881

Purpose

Lentiviral vector encoding RfxCas13d targeting B2M guide

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crB2M-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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PX459v3

Plasmid

#178799

Purpose

SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

Depositor

Paul Thomas

Article

Chey et al BMC Genomics 26, 138 (2025)

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh (Cas9-2A Puro) and U6 (gRNA)

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pC-FLAG-ZCD2

Plasmid

#79274

Purpose

Mammalian expression of ZCD2 with a C-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

ZCD2 (CISD2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits
pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF-1-alpha

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

Plasmid

#196702

Purpose

Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 wild type

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCK033 p5E desma MCS

Plasmid

#195948

Purpose

Gateway p5E vector

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

desmin a regulatory elements (desma Zebrafish)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1

Plasmid

#71858

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

Plasmid

#214878

Purpose

Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crSURF1-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Mito-mGreenLantern

Plasmid

#164461

Purpose

Localization of mGreenLantern fluorescent protein to mitochondria

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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ER-mGreenLantern

Plasmid

#164463

Purpose

Localization and retention of mGreenLantern fluorescent protein in the ER

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Use

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
GFP-IP3KA

Plasmid

#133582

Purpose

Expresses IP3KA GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only