Addgene

We narrowed to 18,124 results for: omp

Showing: 4381 - 4400 of 18124 results

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pJZC545

Plasmid

#62313

Purpose

sgRNA with 1x MS2 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x MS2 binding module

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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Aurein1.2_(+36GFP)-Cre

Plasmid

#71748

Purpose

Aurein1.2+36GFP-Cre fusion expression in E. Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_Aurein1.2+36GFP-Cre

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBT376

Plasmid

#125617

Purpose

Expresses BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTW170b2

Plasmid

#136929

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gVI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

none

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGreen_dualluc_ORF_sensor

Plasmid

#55207

Purpose

Dual-luciferase reporter for microRNA targeting in plants

Depositor

Michael Axtell

Article

Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi:

Inserts

Renilla luciferase
Firefly luciferase

Use

Luciferase; Plant transient expression

Tags

Expression

Mutation

Promoter

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD011

Plasmid

#116852

Purpose

Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
mScarletI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTW211b5

Plasmid

#136927

Purpose

Directed evolution of SpCas9

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9(N)-NpuN

Use

CRISPR

Tags

Expression

Bacterial

Mutation

see manuscript

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-Skp2

Plasmid

#19946

Depositor

Yue Xiong

Article

Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

Insert

S-phase kinase-associated protein 2 (p45) (SKP2 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP RHIM mut

Plasmid

#41385

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant (AAAA-459-462)

Promoter

CMV

Available since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only
psiCHECK SCN1B 3'UTR

Plasmid

#19855

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

SCN1B 3'UTR (SCN1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2

Plasmid

#127127

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pPD159 dsRedExpress2-ZFi43

Plasmid

#138799

Purpose

Constitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

dsRedExpress2-ZFi43

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINK-UBE2S

Plasmid

#66714

Purpose

Expresses UBE2S (1-196) in E. coli

Depositor

David Komander

Article

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Insert

e2-epf (UBE2S )

Use

Tags

GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-NL1A

Plasmid

#15227

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert A (Nlgn1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-PXN

Plasmid

#112957

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

PXN

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAG1242 NLS-mCherry-FIREtag

Plasmid

#205170

Purpose

Expresses NLS-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

NLS-mCherry-FIREtag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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FRB-myc

Plasmid

#20228

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FRB (MTOR Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T2098L point mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only