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pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

H1, 7SK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ADAM7 (wt)

Plasmid

#31139

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM7 (ADAM7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

wt

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/GS-DJ1-D149A-V5

Plasmid

#29345

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Use

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

Asp149Ala

Promoter

Available since

April 20, 2011

Availability

Academic Institutions and Nonprofits only
pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics 26, 138 (2025)

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV eGFP

Plasmid

#206272

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

eGFP

Use

Multimate/gateway entr 4

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV mTagBFP

Plasmid

#206279

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTagBFP

Use

Multimate/gateway entr 4

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AcrIIC1-mCherry chimera #10

Plasmid

#128113

Purpose

Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrIIC1-mCherry chimera #10

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1NhNABP2C

Plasmid

#128312

Purpose

Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP1N+hNABP2C

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_120

Plasmid

#65737

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_144

Plasmid

#65739

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-144

Promoter

T7

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_20_196

Plasmid

#65741

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-196

Promoter

T7

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR™221_RBPMS2

Plasmid

#59389

Purpose

pDONR vector with RBPMS2

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3MX6

Plasmid

#33139

Purpose

gene deletion in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Insert

his3MX6

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKACB_WT

Plasmid

#82224

Purpose

Gateway Donor vector containing PRKACB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKACB (PRKACB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SRC_WT

Plasmid

#82165

Purpose

Gateway Donor vector containing SRC , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SRC (SRC Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KLRC2_WT

Plasmid

#81886

Purpose

Gateway Donor vector containing KLRC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KLRC2 (KLRC2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

N2S; F102S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVtrunc F-tractinN9-39-msfGFPΔN7

Plasmid

#231552

Purpose

Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

Depositor

Manos Mavrakis

Article

Martins et al bioRxiv 2024.04.26.591305

Insert

F-tractin (Itpka Rat)

Use

Tags

msfGFP

Expression

Mammalian

Mutation

Promoter

CMVtrunc

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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