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pMO746

Plasmid

#117480

Purpose

upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

Depositor

Judy Wall

Article

Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

Insert

uracil phosphoribosyltransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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cyto-CFP-FRBx5

Plasmid

#103776

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-CFP-FRBx5

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-cys-His6-OtUBD

Plasmid

#190091

Purpose

Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC2-UMPS-3xFLAG

Plasmid

#87975

Purpose

3rd generation lentiviral vector for Expression of UMPS in mammalian cells

Depositor

David Sabatini

Article

Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

Insert

UMPS (UMPS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-GEX-GECO1

Plasmid

#32443

Purpose

Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

GEX-GECO1.0

Use

Tags

Expression

Mammalian

Mutation

GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
human STIM1 CFP

Plasmid

#19755

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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slc1a3b:myrGFP-P2A-H2AmCherry

Plasmid

#170208

Purpose

Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGFP-P2A-H2AmCherry

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG human DEPTOR

Plasmid

#21334

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N204S and S389N mutations (numbering according to…

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pInsert-PhoCl-RER

Plasmid

#219676

Purpose

Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

CD4mScarlet-PhoCl-RER

Use

Tags

mScarlet and PhoCl

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMIII-Neo-BFP

Plasmid

#153426

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mTagBFP2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGL410_Ins2500

Plasmid

#49058

Purpose

Expresses luciferase under control of mouse insulin 2 gene promoter

Depositor

Kevin Ferreri

Article

Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

Insert

mouse Ins2 promoter (Ins2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ins2 promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 raptor

Plasmid

#21339

Depositor

David Sabatini

Article

Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

Insert

raptor shRNA 1 (Rptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
pHAtC

Plasmid

#78098

Purpose

Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

Depositor

Sang-Gyu Kim, Jin-Soo Kim

Article

Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

U6

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJM600 EF1α PC 3xFLAG-FKBP-FGFR4-TEVp in TUPV2

Plasmid

#161570

Purpose

Constitutive expression of PC 3xFLAG-FKBP-FGFR4-TEVp under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

PC 3xFLAG-FKBP-FGFR4-TEVp

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

EF1α

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-iLIDx6

Plasmid

#103779

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

mCherry-iLIDx6

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB114

Plasmid

#68568

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pUASTattB_NSlmb-vhhGFP4

Plasmid

#35577

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pUAST_NSlmb-vhhGFP4

Plasmid

#35575

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge