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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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KBB285

Plasmid

#185076

Purpose

RFA3-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA3

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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KBB284

Plasmid

#185075

Purpose

RFA2-GFP fusion

Depositor

Ken Belanger

Article

Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

Insert

RFA2

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTSTEP-SNAPf

Plasmid

#72335

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-SNAPf

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAGM9121_TwinStrep-GFP11

Plasmid

#153514

Purpose

TwinStrep-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

TwinStrep-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRS314-prp16-302

Plasmid

#89974

Purpose

prp16-302 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

prp16-302 allele (R456K and G691R)

Promoter

Available since

Aug. 12, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-FKBP-HA no-pA

Plasmid

#82598

Purpose

HA epitope tag fused to C-terminus of human FK506 binding protein 12 (FKBP12) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FKBP-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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His-PaIct

Plasmid

#111293

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

succinyl-CoA:itaconate CoA transferase

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pKM394

Plasmid

#134211

Purpose

Baculovirus expression of CENP-N and CENP-L

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N-his-CENP-L

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSV2-c-erbB2

Plasmid

#16250

Depositor

Mien-Chie Hung

Article

Kwong et al Mol Carcinog. 1998 Oct . 23(2):62-8.

Insert

c-erbB2 (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pSABAD505

Plasmid

#45615

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

GFPN-NpuDnaE_intein-GFP_C

Use

Tags

GFP_C and GFP_N

Expression

Bacterial

Mutation

stemmer GFP with superfolder mutations S30R and Y…

Promoter

pBAD

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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UAS::TVA

Plasmid

#164487

Purpose

Expression of TVA

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Insert

TVA (CD320 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UAS

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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P0050::rpaA (KmR)

Plasmid

#102321

Purpose

drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA with p0050 promoter (+ kanamycin resistance casette)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

-

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLSLZ.D.R

Plasmid

#51510

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI followed by the us:NPTII template. This sequence is between the LIR and SIR.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0026-mCherryBb

Plasmid

#118239

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0026 promoter driven mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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hGR-Dx

Plasmid

#133408

Purpose

overexpression of a mutant human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

N454D, A458T, R460D, D462C

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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RSV CREB M1

Plasmid

#22395

Depositor

Marc Montminy

Article

Gonzalez et al Cell. 1989 Nov 17. 59(4):675-80.

Insert

CREB (Creb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
FLAG-Pol-II 15

Plasmid

#35177

Depositor

Benjamin Blencowe

Article

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Insert

RPBI (POLR2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains the C-terminal repeats 16-52

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only