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Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-BirA-FLAG
Plasmid#105786PurposeExpression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-BirA-FLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1-GFP
Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
UseTags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKEW189-MBP-TEV-AcrVA1
Plasmid#115660PurposeExpresses AcrVA1 in bacteria via T7 RNAP, for purificationDepositorInsertAcrVA1
UseTagsMBP-TEVExpressionBacterialMutationPromoterT7 RNAPAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-Tev-Akt3 WT-2X Flag
Plasmid#87607Purposeexpresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of human Akt3 proteinDepositorInsertAkt3 isoform 2 (AKT3 Human)
UseZebrafish (danio rerio) expressionTags2 tandem flag tags and His-Halo-TevExpressionMammalianMutationAkt3 isoform 2PromoterSP6Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-NDP52
Plasmid#187828PurposePlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586DepositorInsertNDP52 (CALCOCO2 Human)
UseTagsGST-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
UseTags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-FIP200-MBP
Plasmid#171410PurposeFor the purifcation of human ULK1 complexDepositorInsertFIP200 (RB1CC1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-FKBP-TEVp
Plasmid#178867PurposeMammalian expression of mCherry-FKBP-TEVprotease(Δ220-242)DepositorInsertmCherry-FKBP-TEVprotease(Δ220-242)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-DYNC2H1
Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
UseTags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-His6-TEV_SacB
Plasmid#164588PurposeBacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.DepositorInsertTEV-SacB
UseTagsTrx-His6 followed by TEV siteExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-His6-TEV-MMS2
Plasmid#25465DepositorUseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2
Plasmid#174491Purposebacterial expression of human SEPT2DepositorInsertSEPTIN2 (SEPTIN2 Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-C-TEV-Twin-Strep
Plasmid#202527PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site and Twin-strep-tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
Plasmid#137827PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-E
Plasmid#154418PurposeGateway-compatible Entry vectorDepositorInsertE (E SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vector, with insert of e…TagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only