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p3E LAP2b

Plasmid

#105978

Purpose

3' element for Gateway cloning, lamina-associated polypeptide 2

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

LAP2b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP4

Plasmid

#106410

Purpose

P-RAN-RFP4 (RANbody RFP4::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP4

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP2

Plasmid

#106409

Purpose

P-RAN-RFP2 (RANbody RFP2::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP2

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b_GST-Nb32

Plasmid

#228895

Purpose

Express GST-tagged anti-Zika Nb32 in E.coli

Depositor

Magnus Rueping

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

GST-tagged Nb32

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_GST-NbD6

Plasmid

#228896

Purpose

Express GST-tagged anti-Zika NbD6 in E.coli

Depositor

Magnus Rueping

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

GST-tagged NbD6

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJEx411c_pelB-NbD6-Strep2

Plasmid

#228897

Purpose

Periplasmic Expresssion of Strep-tagged anti-Zika NbD6

Depositor

Stefan Arold

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

sb0379

Use

Tags

Strep2

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pJEx411c_pelB-N32-Strep2

Plasmid

#228898

Purpose

Periplasmic Expresssion of Strep-tagged anti-Zika NbD32

Depositor

Stefan Arold

Article

Yuli et al ACS Synthetic Biology Article ASAP

Insert

sb0380

Use

Tags

Strep2

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pET303_sumocodo_GFP_codoopti

Plasmid

#210393

Purpose

Express SUMO-GFP in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

GFP

Use

Tags

SUMO-tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCOLA-Gent-EV

Plasmid

#202491

Purpose

pCOLA-Gent Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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2xMARS-nGFP

Plasmid

#205235

Purpose

Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

Depositor

Jeremy Baskin

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCOLA-Spec-EV

Plasmid

#212095

Purpose

(Empty Backbone) pCOLA-Spec Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-wt7D12

Plasmid

#125268

Purpose

Expresses wild type 7D12 anti-EGFR nanobodies

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 nanobody

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Trc

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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iMbody_pSANG10-3F

Plasmid

#184496

Purpose

Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

Depositor

Marcel Dinger

Article

Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

Insert

iMbody

Use

Tags

3xFLAG, 6xHis, and pelB leader

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2 PCNA GFP

Plasmid

#105937

Purpose

in vitro transcription, cell cycle nuclear marker

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

PCNA (PCNA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Mutation

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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