-
Plasmid#166932PurposeToehold switch part (G)DepositorInsertToehold Switch - Block G - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE-01
Plasmid#166930PurposeStop codon part (E)DepositorInsertSTOP CODON TAA and spacer - BLOCK E- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-01
Plasmid#166925PurposesRNA part (C)DepositorInsertsRNA ToeHold - Block C- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-01
Plasmid#166924PurposePtac promoter part (B)DepositorInsertPTAC promoter - Block B- Flanked by BsaI restriction sites.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pA-01
Plasmid#166923PurposeLacI expression cassette (A)DepositorInsertLacI expression cassette - Block A- Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5C6A-J23106-geneIII
Plasmid#80905PurposePhagemid (encodes cI5C6A and M13 gene III)DepositorInsertsM13 gene III
cI5C6A
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA1-RFP
Plasmid#62247Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA1Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK715
Plasmid#114698PurposeExpress NuMA(1-2115)-RFP-Nano from Rosa 26 locusDepositorInsertNuMA(1-2115)-RFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK746
Plasmid#114712PurposeExpress NuMA(1-2115ΔNLS)5A-3-RFP-Nano from Rosa 26 locusDepositorInsertNuMA(1-2115ΔNLS)5A-3-RFP-Nano
UseTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRoTnsABCDQ
Plasmid#178772PurposeExpression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalisDepositorInsertRotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRoTarget
Plasmid#178774Purposepartial tRNA-Leugene from Rippkaea orientalisDepositorInsertpartial tRNA-Leugene from Rippkaea orientalis
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSth_Cas6
Plasmid#178779PurposeExpression of S. thermophilus type I-E cas6DepositorInsertS. thermophilus type I-E cas6
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-YFP
Plasmid#62245Purposeused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.DepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6G-J23106-geneIII
Plasmid#80906PurposePhagemid (encodes cI5G6G and M13 gene III)DepositorInsertsM13 gene III
cI5G6G
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T-J23106-geneIII
Plasmid#80907PurposePhagemid (encodes cI5G6T and M13 gene III)DepositorInsertsM13 gene III
cI5G6T
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI4A5T6T-J23106-geneIII
Plasmid#80908PurposePhagemid (encodes cI4A5T6T and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T
UseTagsExpressionBacterialMutationPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-AND
Plasmid#62308Purposesynthetic circuit: AND gateDepositorInsertsA1NT sgRNA
A4NT sgRNA
A2NT sgRNA
A1NT
UseCRISPRTagsExpressionBacterialMutationPromoterPA2, PPhlF, pA4, and pBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LI LacZ
Plasmid#104528PurposeLI lacZ moduleDepositorInsertlacZ
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_27
Plasmid#63806Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA4-RFP
Plasmid#62250Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
UseTagsExpressionBacterialMutationPromoterpA4Available sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only