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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_sgEPAS1#1

Plasmid

#183122

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_sgEPAS1#2

Plasmid

#183123

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry_C1_miR21

Plasmid

#65970

Purpose

Expressed mCherry under the control of miR-21 (miR-21 complementary sequence in 3' UTR)

Depositor

Thomas Tuschl

Insert

miR-21 complementary sequence

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_LacZ_sgRNA

Plasmid

#155108

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

LacZ_sgRNA_2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_C292A_mCherry_BamUK

Plasmid

#119761

Purpose

Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-C292A_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated cysteine at position 292 to alanine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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2xmCherry

Plasmid

#127803

Purpose

Homo-dimer mCherry-mCherry expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pZH_mPP7cp_mCherry

Plasmid

#118266

Purpose

Low-noise, tetracycline inducible expression of mPP7cp-mCherry

Depositor

Zach Hensel

Insert

mPP7cp-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLtetO-1

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TEF1pro_mCherry_YFP

Plasmid

#138624

Purpose

Dual reporter backbone plasmid for CCRA experiment

Depositor

Rob Mitra

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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LI B-C mCherry

Plasmid

#54426

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mCherry + 16 aa GS linker

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-DH

Plasmid

#67898

Purpose

Expresses a fusion of mCherry with the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-DH

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_PSMD1_sgRNA_1

Plasmid

#155107

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1 (PSMD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LI C-D mCherry c1

Plasmid

#54418

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mCherry

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry.LIC.HXGPRT

Plasmid

#83364

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locus

Depositor

Vernon Carruthers

Article

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Insert

mCherry

Use

Toxoplasma gondii

Tags

mCherry

Expression

Mutation

Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTec_mCherry

Plasmid

#229145

Purpose

Promoter strength evaluation of Ptec

Depositor

Shrikrishnan Sankaran

Article

Blanch-Asensio et al Microb Cell Fact. 2024 Feb 7;23(1):42. d

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

No

Promoter

Ptec

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_mCherry

Plasmid

#229078

Purpose

Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

Depositor

Bernhard Schmierer

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

EF1s

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry

Plasmid

#229146

Purpose

Promoter strength evaluation of PtlpA

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

No

Promoter

PtlpA

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSU19mCherry

Plasmid

#225181

Purpose

Medium copy cloning vector with mCherry integrated in the multiple cloning site. There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_mCherry

Plasmid

#221470

Purpose

Gateway cloning of mCherry overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

mCherry

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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mhc2dab:mCherry

Plasmid

#68997

Purpose

for making transgenic fish

Depositor

David Traver

Article

Wittamer et al Blood. 2011 Jun 30;117(26):7126-35. doi:

Insert

mhc2dab (mhc2dab Zebrafish)

Use

Zebrafish expression

Tags

mcherry

Expression

Mutation

Promoter

mhc2dab

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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