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Plasmid#125207PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-mut
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionKE
Plasmid#172925PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorKE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_crGE
Plasmid#172933PurposeCrowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crGE
Plasmid#172927PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.DepositorInsertCrowdingSensorGE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRD
Plasmid#172931PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRD
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ionRE
Plasmid#172932PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.DepositorInsertIonicStrengthSensorRE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp173-mCer3-C1
Plasmid#105752Purposea circularly permuted mCerulean3 for FRET sensor optimizationDepositorInsertcp173-mCer3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2-EV-D
Plasmid#125200PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-EV-D
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crE6
Plasmid#172928PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkersDepositorInsertCrowdingSensorE6
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crG18
Plasmid#172930PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 18x GSGDepositorInsertCrowdingSensorG18
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HIFR
Plasmid#192946PurposeLentiviral production of HIF-1alpha reporterDepositorInsertHIF-1alpha reporter
UseLentiviralTagsExpressionMutationPromotermCMVAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crE6G2
Plasmid#172929PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domainDepositorInsertCrowdingSensorE6G2
UseTagsHisExpressionBacterialMutationVal317Gly, Met316GlyPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEmCherry2
Plasmid#118413PurposeRed fluorescent reporter for assessing CRISPR gRNA activityDepositorInsertmCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoC
Plasmid#193809PurposeCMV overexpression of ChemoC in mammalian cell linesDepositorInsertChemoC
UseTags10xHisExpressionMammalianMutationmCerulean3:T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rap1A FLARE
Plasmid#134926PurposeRap1A biosensorDepositorInsertRap1A, mCerulean3, RaIGDS RBD, YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNLuc
Plasmid#208838PurposeExpresses FLAG epitope-tagged CeNLuc "Blue" BRET reporter in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseLentiviralTags1xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pRSETB
Plasmid#48203PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP38-AtLEA4-5
Plasmid#178189PurposeSED1 biosensor for expression in Saccharomyces cerevisiaeDepositorInsertSED1 biosensor
UseTagsExpressionYeastMutationPromoterpPMA1Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Twitch-3 pRSETB
Plasmid#48204PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-3A
UseTags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHBS1449 SED1-Lenti
Plasmid#180824PurposeSED1 biosensor for stable integration and expression in mammalian cellsDepositorInsertSED1 osmosensor
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only