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C. difficile 16S toehold switch sensor

Plasmid

#110704

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

C. difficile 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFX-Sirius-KRasCT

Plasmid

#175831

Purpose

Express Sirius with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-KRasCT

Plasmid

#175832

Purpose

Express SECFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-iRFP-KRasCT

Plasmid

#175836

Purpose

Express iRFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

iRFP with plasma membrane-targeting sequence

Use

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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F. prausnitzii 16S toehold switch sensor

Plasmid

#110705

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

F. prausnitzii 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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R. hominis 16S toehold switch sensor

Plasmid

#110703

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

R. hominis 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
E. rectale 16S toehold switch sensor

Plasmid

#110702

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. rectale 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. breve 16S toehold switch sensor

Plasmid

#110701

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. breve 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. adolescentis 16S toehold switch sensor

Plasmid

#110700

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. longum 16S toehold switch sensor

Plasmid

#110699

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. longum 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B. thetaiotaomicron 16S toehold switch sensor

Plasmid

#110697

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. thetaiotaomicron 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pDL025_HA-rb_IgG_HC_15F11

Plasmid

#193622

Purpose

HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti_HA_15F11 rabbit heavy chain

Use

Tags

signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Article

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Use

Tags

signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

Plasmid

#124703

Purpose

Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E1008Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

Plasmid

#124707

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

Plasmid

#124705

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

Plasmid

#124626

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD (E1008Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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4LNDDE488Q-pCDNA3.1(+)

Plasmid

#172228

Purpose

Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

Depositor

Joshua Rosenthal

Article

Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

Insert

Lambda N peptide - human ADAR2 Deaminase Domain E488Q

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

changed the Glutamic acid 488 in the Deaminase Do…

Promoter

CMV

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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