-
Plasmid#140200PurposeCloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsdLbCas12a crRNA scaffoldExpressionMutationPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pCRI012-pGEM-PacI-PU3-BsmbI-NotI
Plasmid#140204PurposeVector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Step 1 of cloning c…TagsExpressionMutationPromoterAspergillus fumigatus U3 (RNAPIII).Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)
Plasmid#224755PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131C/C159A/C219A/C365A/C399APromoterT7Available sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)
Plasmid#224756PurposeIn vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationS131A/C159A/C219A/C365A/C399APromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS
Plasmid#140193PurposeChromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.DepositorInsertsdLbCas12a-VPR
Bar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
UseCRISPR and Synthetic Biology; Fungal genome integ…TagsVPR (VP64-p65-Rta), NLSExpressionMutationD832A DNAse deactivatedPromoterPgpdA and PtrpCAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRI003-pGEM-LS-Bar-PgpdA-dSpCas9-VPR-TtrpC-LS
Plasmid#140196PurposeChromosomal integration of PgpdA-dSpCas9-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi vector.DepositorInsertsdSpCas9-VPR
Bar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
UseCRISPR and Synthetic Biology; Fungal genome integ…TagsVPR (VP64-p65-Rta) , NLSExpressionMutationPromoterPgpdA and PtrpCAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
Plasmid#83841PurposeFluorescent probe for G1/S transition and M/G1 transitionDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-
Plasmid#169338PurposeExpression of the LT-Fucci(CA) in mammalian cellsDepositorUseTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4
Plasmid#202643PurposeExpresses the LTB4 sensor GEM-LTB4 along with the plasma membrane targeted mKate2 tag in mammalian cellsDepositorInsertPM-mKate2-P2A-GEM-LTB4
UseTagsPM-mKate2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut
Plasmid#202644PurposeExpresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cellsDepositorInsertPM-mKate2-P2A-GEM-LTB4mut
UseTagsPM-mKate2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)
Plasmid#169336PurposeExpression of the LT-Fucci(SA) in mammalian cellsDepositorUseTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+
Plasmid#169337PurposeExpression of the LT-Fucci(SCA) in mammalian cellsDepositorUseTagsHaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert
Plasmid#111153Purposeknock in bait for Axolotl Pax7 locusDepositorInsertGAP43-Cherry-T2a-mERt-Cre-Ert
UseCre/LoxTagsExpressionMutationwtPromoterAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP
Plasmid#205762PurposeSingle-color FUCCI4 senor for transitions of all four cell cylce phasesDepositorInsertrsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast
Plasmid#193139PurposeExpresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistanceDepositorUseLentiviralTagsExpressionMutationhuman Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…PromoterEF1aAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119067PurposeDonor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertNLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
Plasmid#119065PurposeDonor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombinationDepositorInsertHA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only