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Plasmid#178421PurposeEmpty backbone. T7Max promoter with N-His TagDepositorTypeEmpty backboneUseSynthetic BiologyTags8xHisExpressionBacterialMutationPromoterT7MaxAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCTCON2-GXB
Plasmid#160926PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)DepositorInsertsBFP
sfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-Aga1p-FAPB2.3.6L1TAG
Plasmid#158143PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertFAPB2.3.6L1TAG
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT3928
Plasmid#113331Purposecontrol for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoterDepositorInsertsbeta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHBA03
Plasmid#83537PurposeConstitutive TEF1 promoter mediated expression of the UBiC, ARO4*, and TKL1 genesDepositorInsertpTEF1-UBiC-CYC1t-pTEF1-ARO4*-CYC1t-pTEF1-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas73
Plasmid#82398PurposeΔccm:Km, used to delete ccm operonDepositorInsertccm deletion
UseTagsExpressionMutationPromoternoneAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
UseTagsExpressionYeastMutationPromoterGPD1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS2
Plasmid#70135PurposeCEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertDS2
UseSynthetic BiologyTagsExpressionMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbB0a-RFP
Plasmid#35371DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB0k-RFP
Plasmid#35370DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbB0c-RFP
Plasmid#35366DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbS3c-RFP
Plasmid#35311DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterProSAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBbE3c-RFP
Plasmid#35305DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterProSAvailable sinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK793
Plasmid#8827DepositorInsertTEV protease, S219V mutant
UseTagsHis, MBP (with TEV), and polyarginineExpressionBacterialMutationS219V mutation (improves stability)PromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Actinin-sstFRET-GR
Plasmid#83416PurposeFRET-based actinin tension sensorDepositorInsertActinin-sstFRET-GR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only