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Plasmid#197845PurposeDonor vector to restore L1 destination via Esp3I (reverse orientation)DepositorInsertNone
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-MED6-mEmerald donor
Plasmid#197865PurposeDonor plasmid used for building MED6-mEmerald knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pminiT-mEmerald-MED1 donor
Plasmid#197867PurposeDonor plasmid used for building mEmerald-MED1 knock-in mESCsDepositorInsertCRSP210 (Med1 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript II SK-SNAP donor for Hist1h3g
Plasmid#186932PurposeGenomic targeting of SNAP tag at Hist1h3g C-terminalDepositorInsertSNAP donor for Hist1h3g (H3c8 Mouse)
UseTagsSNAPExpressionMammalianMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:myc-GluA2 TKIT donor
Plasmid#169423PurposeDonor DNA for TKIT knockin of Myc to GluA2 in mouseDepositorInsertMyc (Myc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC_cherry donor_Rosa target (pE002)
Plasmid#149348PurposeReplace donor vector for cherry insertion using the mouse Rosa26 guide RNADepositorInsertcherry
UseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC_cherry donor_Polb target (pE038)
Plasmid#149349PurposeReplace donor vector for cherry insertion using the human Polb guide RNADepositorInsertcherry
UseTagsExpressionMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
HoxC-downstream-5xTetO Donor
Plasmid#131341PurposeDonor template to insert 5xTet operator sequence into downstream of HoxC cluster.DepositorInsertHoxC-downstream-5xTetO Donor
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor CFP-hDF
Plasmid#72590PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGDF8 donor CFP-hDF
Plasmid#72591PurposePlasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-human-p16INK4A-P2A-mCherry
Plasmid#137026Purposedonor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4ADepositorInsertp16INK4A-knockin-homology arm-P2A-mCherry
UseTagsP2A-mCherryExpressionBacterialMutationPromoterno promoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ6332 - ACTB CRISPRa CLIP Donor
Plasmid#210015PurposeCLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locusDepositorInsertdLbCpf1-miniVPR-T2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-EGFP HR donor vector
Plasmid#137925PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorInsertSA-H2B-EGFP-bpA with homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-mCherry HR donor vector
Plasmid#137928PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorInsertSA-H2B-mCherry-bpA with homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterhPGK1Available sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-donor_opt(R2Tg)-GFP(intron)
Plasmid#223252PurposeMammalian expression of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo (Universal)
Plasmid#80767PurposeUniversal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorInsertPITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX-N-CTCF-mAID-Halo-donor
Plasmid#139747PurposeMouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTagDepositorInsertCTCF CRISPR knock-in (Ctcf Mouse)
UseCRISPRTagsmAID-HaloTagExpressionMutationPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gata3-T2A-H2B-eGFP donor
Plasmid#113119PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequenceDepositorInsertGata3 donor region including homology arms and T2A-H2B-eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-KRAB-Hygro donor
Plasmid#199621PurposeDonor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-idCas9-KRAB-Hygro
UseTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only