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Plasmid#201126PurposepLEX_307 plasmid with N-terminal mKate tag and zeo markerDepositorTypeEmpty backboneUseTagsmKateExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only
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plex_307-mKate-ccdB-g418
Plasmid#201125PurposepLEX_307 plasmid with N-terminal mKate tag and g418 markerDepositorTypeEmpty backboneUseTagsmKateExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
plex_307-3xflag-ccdb-blasticidin
Plasmid#201123PurposepLEX_307 plasmid with N-terminal FLAG tag and blasticidin markerDepositorTypeEmpty backboneUseTags3xFLAGExpressionMammalianMutationPromoterEF-1αAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pANY4-pR18/pL18-ccdB
Plasmid#164899PurposeTwo valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pR18/pL18-ccdB.DepositorInsertccdB cassette
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-EBFP2-ccdB-EYFP
Plasmid#158558PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH9-ilvA-ccdBwt (DE25)
Plasmid#135170PurposeExpressing synthetic overlapping gene, ilvA-ccdBDepositorInsertilvA-ccdB
UseSynthetic BiologyTagsExpressionMutationPromoterTetOAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-AtU3b-tRNA-ccdB-gRNA
Plasmid#118390PurposeEntry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertAtU3b promoter
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin
Plasmid#115519PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertccdB-sgRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEFS-ccdB-12X MS2_mPGK-Puro
Plasmid#177809PurposeAssembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'DepositorTypeEmpty backboneUseTags12X MS2 stem loopsExpressionMammalianMutationPromoterEFSAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB-SNAP
Plasmid#166148PurposeGateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.DepositorArticleTypeEmpty backboneUseTagsHis10-Smt3 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA
Plasmid#186407PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA
Plasmid#186410PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-ccdB-boxB-tBE-V5-mA3
Plasmid#171693PurposeExpresses tBE-V5-mA3 in mammalian cellsDepositorInsertccdB-sgRNA scaffold-boxB-tBE-V5-mA3
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and EF1aAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA
Plasmid#186408PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP
Plasmid#186412PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus
Plasmid#186409PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2
Plasmid#186414PurposeAttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterBasal promoter of the FOG geneAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro
Plasmid#173228PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only