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pCS2MT-mbeta-catenin delta5

Plasmid

#100882

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 400-666aa

Promoter

CMV

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta2

Plasmid

#100879

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 1-276aa

Promoter

CMV

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2MT-mbeta-catenin delta1

Plasmid

#100878

Purpose

Mammalian expression of mouse beta-catenin truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

Ctnnb1 (Ctnnb1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

truncated Ctnnb1 1-150aa

Promoter

CMV

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1

Plasmid

#48970

Purpose

Expresses catalytic domain of MALT1 in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566

Promoter

T7

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pPiggyBacATCC LacZ GCTT loxAttP

Plasmid

#173496

Purpose

piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+ MT Beta Catenin (Xenopus)

Plasmid

#13435

Depositor

Marc Kirschner

Article

Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

Insert

Beta Catenin (ctnnb1.L Frog)

Use

Tags

Myc Tag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4

Plasmid

#112844

Purpose

transient/stable overexpression of the N-terminal and central domains of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

N + C1 domains of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFS331-USP44-catDead-HIS

Plasmid

#43903

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

C282A

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-2

Plasmid

#112842

Purpose

transient/stable overexpression of the central domain of Catulin.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Catulin-alpha-1 (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

C1 domain of Catulin

Promoter

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM beta catenin (CT#593)

Plasmid

#13938

Depositor

Cliff Tabin

Article

Hartmann et al Development. 2000 Jul . 127(14):3141-59.

Insert

beta catenin (CTNNB1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Amino acids 64-272

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pDEST14-SpyCatcher003-TEVs-SpyTag003DA

Plasmid

#216283

Purpose

Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SpyCatcher003-TEVs-SpyTag003DA

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST14-Cys-SpyCatcher (Min)

Plasmid

#216713

Purpose

Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

Depositor

Alex Dunn

Article

Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

Insert

Cys-SpyCatcher (Min)

Use

Tags

6xHis

Expression

Bacterial

Mutation

See Li...Howarth, J. Mol. Biol., 2014

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b 6His SpyCatcher-GRFT

Plasmid

#204502

Purpose

Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

Depositor

Urartu Seker

Article

Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

Insert

GRFT

Use

Synthetic Biology

Tags

6xHis and SpyCatcher

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAB-EXPR(PYL-cat_dom_HDAC5)

Plasmid

#114401

Purpose

For PYL-HDAC5 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 catalytic domain

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

S220F and the deletion of M221

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET24-aCatenin(82-906)-His6

Plasmid

#203332

Purpose

Bacterial expression of wild-type alpha-catenin

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 82-906

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-p450-hcvs-SpyCatcher

Plasmid

#179677

Purpose

HCVP-cleavable ER-associated SpyCatcher

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

HCVP SpyCatcher

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACE-Truncated-Kir3.2-StreptagII

Plasmid

#177263

Purpose

Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

Depositor

Arthur Laganowsky

Article

Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

Insert

G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)

Use

Tags

StreptagII

Expression

Insect

Mutation

Residues 49-378

Promoter

polyhedrin promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-Zdk1-Vav2cat

Plasmid

#178875

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to mCherry-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

mCherry-Zdk1-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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