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Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Capping Protein
Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsUseTags6*HisExpressionBacterialMutationV222IPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60B
Plasmid#48740PurposeBacterial expression of UNC-60B (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60B cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60A
Plasmid#48739PurposeBacterial expression of UNC-60A (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60A cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-C
Plasmid#118372PurposeGateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-N
Plasmid#118373PurposeGateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-N
Plasmid#118371PurposeGateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Arp11 (1-417)
Plasmid#51398Purposeexpression of full-length mouse Arp11 in mammalian cellsDepositorInsertArp11 (Actr10 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-Full Length (aa1-564)-EGFP
Plasmid#187692PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-His-GFP-Arp11
Plasmid#51402Purposeexpression of GFP-tagged mouse Arp11 under T7 promoterDepositorInsertArp11 (Actr10 Mouse)
UseTagsGFP and HisExpressionBacterialMutationPromoterT7Available sinceMarch 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-His-Arp11(1–417)
Plasmid#51400Purposeexpression of full-length mouse Arp11 under T7 promoterDepositorInsertArp11 (Actr10 Mouse)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-E (aa1-138)-EGFP
Plasmid#187697PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa1-138PromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-G (aa1-372)-EGFP
Plasmid#187699PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa1-372PromoterCMVAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-F (aa373-564)-EGFP
Plasmid#187698PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa373-564PromoterCMVAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-C (aa139-371)-EGFP
Plasmid#187695PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa139-371PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-A (aa139-564)-EGFP
Plasmid#187693PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa139-564PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-D (aa160-340)-EGFP
Plasmid#187696PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa160-340PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-B (aa309-564)-EGFP
Plasmid#187694PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa309-564PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only