Skip to main content
Addgene
Showing: 421 - 440 of 24298 results

  3. pJKW 0643

    Plasmid
    #74121
    Purpose
    Photinus pyralis luciferin sulfotransferase LST - pHis8-4
    Depositor
    Jing-Ke Weng
    Article
    Fallon et al Biochemistry. 2016 May 26.
    Insert
    Photinus pyralis firefly luciferin sulfotransferase
    Use
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74121-plasmid-map-sequence-id-138912

    Enlarge

  4. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid
    #178320
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi
    Inserts
    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    178320-plasmid-map-sequence-id-363290

    Enlarge

  5. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid
    #124536
    Purpose
    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1
    Depositor
    Koen Venken
    Article
    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1
    Inserts
    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    124536-plasmid-map-sequence-id-272178

    Enlarge

  6. CMV-LUC2CP/intron/ARE

    Plasmid
    #62858
    Purpose
    splicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    insertion of chimeric intron at nueotide poclsiti…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62858-plasmid-map-sequence-id-243538

    Enlarge

  7. pJKW 0633

    Plasmid
    #74122
    Purpose
    Photinus pyralis sulfotransferase ST2 - pHis8-4
    Depositor
    Jing-Ke Weng
    Article
    Fallon et al Biochemistry. 2016 May 26.
    Insert
    Photinus pyralis sulfotransferase 2
    Use
    Tags
    8x-His, TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74122-plasmid-map-sequence-id-142571

    Enlarge

  8. pJKW 0690

    Plasmid
    #74123
    Purpose
    Photinus pyralis sulfotransferase ST3 - pHis8-4
    Depositor
    Jing-Ke Weng
    Article
    Fallon et al Biochemistry. 2016 May 26.
    Insert
    Photinus pyralis sulfotransferase 3
    Use
    Tags
    8x-His,TEV protease site (ENLYFQ)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74123-plasmid-map-sequence-id-142447

    Enlarge

  10. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

    Enlarge

  11. pDLucBiDi

    Plasmid
    #219562
    Purpose
    Dual luciferase plasmid with Renilla luciferase constitutively expressed and firefly luciferase downstream of MCS without a start codon
    Depositor
    Pasha Baranov
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40 and T7
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    219562-plasmid-map-sequence-id-439245

    Enlarge

  12. pGL3_MSR1_bothneg0topos3pos1

    Plasmid
    #232387
    Purpose
    MSR1 enhancer, left buffering Coordinator converted to sensitizing double Ebox and right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, left buffering Coordinator convert…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232387-plasmid-map-sequence-id-462867

    Enlarge

  15. pEPCTΩSP0022

    Plasmid
    #187562
    Purpose
    Module for copper-inducible expression of nanoluc luciferase and firefly luciferase driven by minimal synthetic promoters with binding sites for CUP2
    Depositor
    Nicola Patron
    Article
    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.
    Insert
    Firefly Luciferase and NanoLuciferase
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    187562-plasmid-map-sequence-id-368364

    Enlarge

Showing: 421 - 440 of 24298 results