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Flag-SKP1-RR (K142R/K163R)

Plasmid

#213425

Purpose

Expresses human SKP1 K142R/K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R/K163R (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-F139A/I141A

Plasmid

#213687

Purpose

Expresses human SKP1-F139A/I141A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-F139A/I141A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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6MIW

Plasmid

#127291

Purpose

Bacterial expression for structure determination. May not contain entire coding region of gene

Depositor

Cheryl Arrowsmith

Article

Munzker et al Commun Biol. 2024 Jul 24;7(1):901. doi:

Insert

HUWE1 (HUWE1 Human)

Use

Tags

6xHis-TEV (MHHHHHHSSGRENLYFQG)

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX4T1-TEV-HsFbxo7-129-398/Skp1

Plasmid

#202592

Purpose

Bacterial expression of HsFbxo7-129-398 and HsSkp1

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Inserts

Fbxo7 (FBXO7 Human)
Skp1 (SKP1 Human)

Use

Tags

GST, TEV

Expression

Bacterial

Mutation

aa 129-398 only

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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KHBD00534

Plasmid

#39632

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

Plasmid

#228530

Purpose

Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Synthetic, Photinus pyralis)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-FBXL5-SKP1

Plasmid

#226620

Purpose

Co-expression of FBXL5-SKP1 complex in E.coli

Depositor

Ning Zheng

Article

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Inserts

FBXL5 (FBXL5 Human)
SKP1 (SKP1 Human)

Use

Tags

GB1

Expression

Bacterial

Mutation

Amino acids 1-198 was deleted, and 420-596 was re…

Promoter

T7

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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KHBD00450

Plasmid

#39563

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00053

Plasmid

#34215

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pLD-puro-Cc-PRDX5WT-VA

Plasmid

#98688

Purpose

Lentiviral expression of human PRDX5-WT in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-GRK [N145/20R]

Plasmid

#206536

Purpose

Mammalian Expression Plasmid of anti-Pan-GRK (Mouse). Derived from hybridoma N145/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-GRK (Mus musculus) recombinant Mouse monoclonal antibody (Grk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K85R

Plasmid

#98694

Purpose

Gateway cloning of human PRDX5-K85R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K85R

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K83R

Plasmid

#98693

Purpose

Gateway cloning of human PRDX5-K83R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K83R

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5E80K

Plasmid

#98692

Purpose

Gateway cloning of human PRDX5-E80K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E80K

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5K85R-VA

Plasmid

#98691

Purpose

Lentiviral expression of human PRDX5-K85R in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

K85R

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-PRDX5E80K-VA

Plasmid

#98689

Purpose

Lentiviral expression of human PRDX5-E80K in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

E80K

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD-puro-Cc-PRDX5K83R-VA

Plasmid

#98690

Purpose

Lentiviral expression of human PRDX5-K83R in mammalian cells

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Lentiviral

Tags

VA tag

Expression

Mammalian

Mutation

K83R

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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