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Plasmid#159013PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0384
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0229:Dendra
Plasmid#159005PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0229
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0024:Dendra
Plasmid#158997PurposeT7 expression of C-term fluorescent fusion in E. coliDepositorInsertMSMEG_0024
UseIntegrating mycobacterial expression vector attl5TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialMutationPromoterpmycTetOAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPV-DsRed2-ER-5
Plasmid#170571PurposeExpresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-ER-5
Plasmid#170570PurposeExpresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-FLAG-ER-5
Plasmid#170622PurposeExpresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.DepositorInsertAPV-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-FLAG-DsRed2-ER-5
Plasmid#170620PurposeExpresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.DepositorInsertAPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPV-FLAG-DsRed2-ER-5
Plasmid#170621PurposeExpresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.DepositorInsertEPV-FLAG-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-mEmerald-DsRed2-ER-5
Plasmid#170681PurposeExpresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.DepositorInsertAPV-mEmerald-DsRed2-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPV-DsRed2-mEmerald-ER-5
Plasmid#170715PurposeExpresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.DepositorInsertAPV-DsRed2-mEmerald-ER-5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC2-G-GECO1.2
Plasmid#207141PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC2-D276K-G-GECO1.2
Plasmid#207142PurposeFusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationChanged Aspartate 276 to LysinePromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-TagBFP-DiB-RM
Plasmid#168782PurposeActin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RMDepositorInsertLifeAct-TagBFP-DiB-RM
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmEmerald-ERGIC-53
Plasmid#170717PurposeExpresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.DepositorInsertERGIC-53 (LMAN1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRPML1-G-GECO1.2-ERES
Plasmid#207144PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signalDepositorInsertMcoln1 (MCOLN1 Human)
UseTagsER-Export Signal (ERES) and G-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only