Skip to main content
Addgene
Showing: 421 - 440 of 443 results

  3. pAPV-DsRed2-ER-5

    Plasmid
    #170570
    Purpose
    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170570-plasmid-map-sequence-id-335779

    Enlarge

  4. pAPV-DsRed2-FLAG-ER-5

    Plasmid
    #170622
    Purpose
    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170622-plasmid-map-sequence-id-335778

    Enlarge

  5. pLCS105

    Plasmid
    #209358
    Purpose
    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    209358-plasmid-map-sequence-id-420623

    Enlarge

  6. pJRA105

    Plasmid
    #209338
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal Dendra2 fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209338-plasmid-map-sequence-id-420459

    Enlarge

  7. pAPV-FLAG-DsRed2-ER-5

    Plasmid
    #170620
    Purpose
    Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-FLAG-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170620-plasmid-map-sequence-id-335780

    Enlarge

  9. pAPV-mEmerald-DsRed2-ER-5

    Plasmid
    #170681
    Purpose
    Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-mEmerald-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170681-plasmid-map-sequence-id-335805

    Enlarge

  10. pAPV-DsRed2-mEmerald-ER-5

    Plasmid
    #170715
    Purpose
    Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-mEmerald-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170715-plasmid-map-sequence-id-335807

    Enlarge

  13. pEG584

    Plasmid
    #40191
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. pEG587

    Plasmid
    #40196
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S458A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only

  15. pEG585

    Plasmid
    #40192
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    S404A; exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  16. pEG634

    Plasmid
    #40121
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa244-468) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa244-468
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only

  19. pEG610

    Plasmid
    #40195
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
    Use
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    contains aa 1-355; exon 2 recoded to be RNAi-resi…
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only

  20. pEG593

    Plasmid
    #40119
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
    Use
    Gateway destination vector
    Tags
    Dendra2 PAFP (sequence optimized for worm express…
    Expression
    Worm
    Mutation
    R274E, K318E, Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available since
    Oct. 4, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 421 - 440 of 443 results